We narrowed to 19,663 results for: Met

Showing: 10261 - 10280 of 19663 results

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FH-TET3-HxDmut-pEF

Plasmid

#127896

Purpose

Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079A

Depositor

Patrick Hogan, Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

TET3-HxDmut (TET3 Human)

Tags

HA and Flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-shFyn

Plasmid

#26985

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

shRNA Fyn (FYN Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pBait

Plasmid

#165146

Purpose

Bait plasmid for PROBER (empty backbone encoding replaceable ccdB cassette)

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET-21a-PE2-His

Plasmid

#201973

Purpose

Express His-tagged PE2 in bacteria

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

PE2-HIS

Tags

His and c-myc NLS

Expression

Bacterial

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSprayerHE

Plasmid

#165150

Purpose

Sprayer plasmid for PROBER encoding BASU-GAL4 fusion (High Expression)

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

BASU-GAL4

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-PCNA-IRES-puro2b

Plasmid

#26461

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

PCNA (PCNA Human)

Tags

pEGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pTK88_GFP-Anillin

Plasmid

#46354

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

Anillin (ANLN Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

a1854 is changed to c to disrupt XhoI site withou…

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pBDC

Plasmid

#135188

Purpose

Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

Depositor

Hongqing Fang

Article

Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

Insert

CmR

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Promoter

cat

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+-H2B-pr-mEosFP

Plasmid

#141101

Purpose

H2B fused to pr-mEosFP for chromatin visualization and primed conversion

Depositor

Periklis Pantazis

Article

Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

Insert

Histone 2B

Tags

pr-mEosFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSLHDR02_EGFP_tCD8

Plasmid

#124061

Purpose

Construction of 1st donor for scarless genome editing

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Inserts

EGFP
tCD8

Use

Mouse Targeting; Human targeting

Promoter

Human UbC promoter modified

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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2xP53_RE::FLuc

Plasmid

#178316

Purpose

P53 Firefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

P53 FLuc Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP1055

Plasmid

#119242

Purpose

construction intermediate

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Available Since

Dec. 27, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-iSH2

Plasmid

#66839

Purpose

Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

iSH2

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::CreERT2-bGHpA

Plasmid

#62444

Purpose

MXS_chaining vector with PGK::CreERT2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-mRFP1

Plasmid

#53745

Purpose

Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

mRFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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PL-SIN-PGK-EiP

Plasmid

#21312

Purpose

Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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Tubulin-hfYFP

Plasmid

#186515

Purpose

Localization of hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Tubulin

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Plasmid

#219782

Purpose

CaST-IRES for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-hfYFP

Plasmid

#191201

Purpose

Constitutive CAG-driven expression of hfYFP in animals using AAV

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Use

AAV

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CAG

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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