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pLKO.1 - TRC cloning vector

Plasmid

#10878

Purpose

3rd generation transfer plasmid

Depositor

David Root

Article

Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#69496

Purpose

pJL1 expression vector with sfGFP

Depositor

Michael Jewett

Article

Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

Insert

Super folder GFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO mTRPM7

Plasmid

#45482

Depositor

Andrew Scharenberg

Article

Nadler et al Nature. 2001 May 31;411(6837):590-5.

Insert

TRPM7 (Trpm7 Mouse)

Use

Tetracycline inducible

Tags

Flag

Expression

Mammalian

Promoter

Tetracycline-controlled CMV

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3.1(+)-CMV-bArrestin2-TEV

Plasmid

#107245

Purpose

Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ARRB2

Tags

TEV(NIA), tobacco etch virus NIA

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pALB-GFP

Plasmid

#55759

Purpose

Fluorescent marker for hepatocytic lineage

Depositor

Snorri Thorgeirsson

Article

Heo et al Hepatology. 2006 Dec;44(6):1478-86.

Insert

albumin enhancer/promoter (Alb Mouse)

Expression

Mammalian

Promoter

Mouse Albumin Enhancer/Promoter

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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CMV-Hylight-T152E

Plasmid

#193448

Purpose

A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)

Depositor

Richard Goodman

Article

Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Binding-Dead Hylight

Expression

Mammalian

Mutation

Threonine 67 (ACT) mutated to a Glutamic Acid (GA…

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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p5E-mpeg1.1

Plasmid

#75023

Purpose

Can be used to drive expression in zebrafish microglia.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

mpeg1.1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Murine E-cadherin mCherry

Plasmid

#71366

Purpose

mammalian expression of murine E-caderin with mCherry fusion

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

E-cadherin (Cdh1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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CanlonicSF

Plasmid

#178342

Purpose

To explore lactate dynamics in intact systems

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

CanlonicSF

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Flag Intersectin Short (human)

Plasmid

#47392

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

Intersectin short (ITSN1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1 - TRC control

Plasmid

#10879

Purpose

Negative control shRNA vector containing non-hairpin insert.

Depositor

David Root

Article

Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

Insert

non-hairpin 18bp

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 WT

Plasmid

#227190

Purpose

Inducible lentiviral expression of WT APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-his-BACH1 WT

Plasmid

#17642

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pLIX403 RNaseH1 Catalytic Inactive

Plasmid

#237796

Purpose

Inducible lentiviral expression of RNaseH1 Catalytic Inactive

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

RNaseH1 (RNASEH1 Human)

Expression

Mammalian

Mutation

D210N

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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HA-BirA*-pDEST-N-pcDNA5/FRT/TO

Plasmid

#118375

Purpose

Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-BirA*

Expression

Mammalian

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1 SA-2A-puro-pA donor

Plasmid

#22075

Purpose

AAV zinc finger donor vector for genomic targeting

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-Puro

Use

Zfn donor

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X

Plasmid

#192151

Purpose

T cell CRISPR AAV-SB vector

Depositor

Sidi Chen

Article

Ye et al Nat Biotechnol. 2019 Nov;37(11):1302-131

Type

Empty backbone

Use

AAV

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

Plasmid

#221080

Purpose

SEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X-GS3

Plasmid

#204745

Purpose

Expression of Cas9 and human H2AX

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX (H2AX Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4/hL3MBTL2

Plasmid

#28232

Depositor

Danny Reinberg

Article

Trojer et al Cell. 2007 Jun 1. 129(5):915-28.

Insert

l(3)mbt-like 2 (Drosophila) (L3MBTL2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only