165,637 results

1. pFA6a URA3

   Plasmid

   #61924

   Purpose

   Expression of S. cerevisiae URA3 cloned into pFA6a

   Depositor

   Jon Houseley, David Tollervey

   Article

   Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

   Insert

   URA3
   

   Use

   Yeast genomic targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-Sec61β

   Plasmid

   #172445

   Purpose

   Expression of ER protein Sec61beta with an N-terminal mCherry tag

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Protein transport protein Sec61 subunit beta
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV5-Flag-RhoA wt

   Plasmid

   #11750

   Depositor

   Jeff Wrana

   Article

   Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

4. SpCas9-TadCBEd-ivt-template

   Plasmid

   #193843

   Purpose

   Template for in vitro transcription of TadCBEd (with SpCas9)

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEd with SpCas9
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen-ETSSB

   Plasmid

   #165549

   Purpose

   ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   Extreme Thermostable Single-Stranded DNA Binding Protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAG153 FKBP-CFAST11

   Plasmid

   #130813

   Purpose

   Expresses FKBP-CFAST11 in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

   Insert

   FKBP-CFAST11
   

   Use

   Tags

   Myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. XLone-Puro eGFP

   Plasmid

   #140027

   Purpose

   Tunable and temporal expression control of nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.Syn.GCaMP6s.WPRE.SV40

   Plasmid

   #100843

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEQ176

   Plasmid

   #83943

   Purpose

   express beta-galactosidase in eukaryotic cells

   Depositor

   Adam Geballe

   Article

   Schleiss et al J Virol. 1991 Dec;65(12):6782-9.

   Insert

   CMV enhancer/promoter
   

   Use

   Tags

   beta-galactosidase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0208

    Plasmid

    #142556

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXP2 (FOXP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV 3xFLAG-GABARAP WT

    Plasmid

    #123097

    Purpose

    Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. NanoLuc-PM

    Plasmid

    #164784

    Purpose

    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
    

    Use

    Tags

    NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRESneo-FLAG/HA Ago2 corrected

    Plasmid

    #10822

    Depositor

    Thomas Tuschl

    Article

    Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    3 silent mutations in orf

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH47751

    Plasmid

    #48002

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pIRE4-Bpa

    Plasmid

    #155342

    Purpose

    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

    Depositor

    Irene Coin

    Article

    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

    Insert

    humanized EBpaRS/BstYam
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/U6

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmiRFP713-N1

    Plasmid

    #136559

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP713

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP713
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR_TetO_CD19-P2A-mCherry

    Plasmid

    #133803

    Purpose

    repressible expression of CD19 and mCherry in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2018 Jul 13;361(6398):156-162.

    Insert

    putative ECD of H. sapiens CD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. G6PD/pRK5

    Plasmid

    #41521

    Depositor

    Xiaolu Yang

    Article

    Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

    Insert

    glucose-6-phosphate dehydrogenase (G6PD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-L1-PGK-PuroTK-L2

    Plasmid

    #141005

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    PuroTK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-AKT-PH-GFP

    Plasmid

    #18836

    Depositor

    Craig Montell

    Article

    Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

    Insert

    AKT-PH domain (AKT1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains amino acids 1-148 (compared to GenBank r…

    Promoter

    CMV

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3

    Plasmid

    #200630

    Purpose

    Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing pad

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    iCasp9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pTRE3G

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSD080A

    Plasmid

    #218138

    Purpose

    Lentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD40-eGFP-IRES2-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAV-mOXT-hM3Dq-mCherry-WPRE

    Plasmid

    #70717

    Purpose

    Expresses DREADDs Gq receptor under oxytocin promoter control

    Depositor

    Daniel Geschwind

    Article

    Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    mouse oxytocin

    Available since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3.1-V5-PTK2B

    Plasmid

    #127233

    Purpose

    Eukaryotic expression of PTK2B

    Depositor

    Kai Johnsson

    Article

    Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

    Insert

    PTK2B
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA5/FRT/TO_HaloTag7_T2A_EGFP

    Plasmid

    #169325

    Purpose

    Expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLentiCRISPR v2-sgControl

    Plasmid

    #230080

    Purpose

    Crispr knock out control

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    non-specific sequence control
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSpCas9_T2A_mCherry sgBFP

    Plasmid

    #192929

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAMD1-6

    Plasmid

    #61566

    Purpose

    Standardized mini-Tn5 delivery plasmid, gentamicin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CP1028-ABE8e

    Plasmid

    #138492

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9 (CP1028)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-teLuc c-myc

    Plasmid

    #100026

    Purpose

    Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

    Depositor

    Huiwang Ai

    Article

    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

    Insert

    teLuc, a NanoLuc mutant
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    D19S, D85N, C164H

    Promoter

    CMV

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge