334,986 results

1. pLKO.1 GFP shRNA

   Plasmid

   #30323

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   GFP shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1 GFP shRNA

   Plasmid

   #30323

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   GFP shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hLRP1-pEZT-BM

   Plasmid

   #177985

   Purpose

   Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

   Depositor

   Lora Hooper

   Article

   Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

   Insert

   LDL receptor related protein 1 (LRP1 Human)
   

   Tags

   No

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. hLRP1-pEZT-BM

   Plasmid

   #177985

   Purpose

   Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

   Depositor

   Lora Hooper

   Article

   Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

   Insert

   LDL receptor related protein 1 (LRP1 Human)
   

   Tags

   No

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-N-Myc_WT USP7

   Plasmid

   #131242

   Purpose

   Mammalian expression of N-terminally Myc-tagged USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV enhancer + CMV promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-N-Myc_WT USP7

   Plasmid

   #131242

   Purpose

   Mammalian expression of N-terminally Myc-tagged USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV enhancer + CMV promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBa-LSS-GFP-LDLR wt

   Plasmid

   #98184

   Purpose

   Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

   Insert

   LDLR (LDLR Human)
   

   Tags

   GFP and LDLR signal sequence, N-term of GFP so GF…

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Chicken Beta Actin

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBa-LSS-GFP-LDLR wt

   Plasmid

   #98184

   Purpose

   Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

   Insert

   LDLR (LDLR Human)
   

   Tags

   GFP and LDLR signal sequence, N-term of GFP so GF…

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Chicken Beta Actin

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. eSpCas9(1.1)

   Plasmid

   #71814

   Purpose

   Expresses high specificity SpCas9. Px330-like plasmid.

   Depositor

   Feng Zhang

   Article

   Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

   Has Service

   Cloning Grade DNA

   Insert

   enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. eSpCas9(1.1)

    Plasmid

    #71814

    Purpose

    Expresses high specificity SpCas9. Px330-like plasmid.

    Depositor

    Feng Zhang

    Article

    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

    Has Service

    Cloning Grade DNA

    Insert

    enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pWM_12x601_25bpLinker

    Plasmid

    #157788

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_25bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWM_12x601_25bpLinker

    Plasmid

    #157788

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_25bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Ef1a-mCherry-IRES-Cre

    Plasmid

    #55632

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Inserts

    mCherry
    Cre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-Ef1a-mCherry-IRES-Cre

    Plasmid

    #55632

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Inserts

    mCherry
    Cre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7-EGFP-C1-HsEDC3

    Plasmid

    #25032

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    EDC3 (EDC3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-EGFP-C1-HsEDC3

    Plasmid

    #25032

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    EDC3 (EDC3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIR-CMV-SCN2A-Variant-4-IRES-mScarlet

    Plasmid

    #209410

    Purpose

    Expression of wild-type neonatal isoform of human SCN2A.

    Depositor

    Al George

    Article

    Thompson et al bioRxiv. 2023 Feb 23:2023.02.23.529757.

    Insert

    SCN2A Variant 4, stabilized (SCN2A Human)
    

    Expression

    Mammalian

    Mutation

    IVS intron inserted after bp 4551 of the SCN2A OR…

    Promoter

    CMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIR-CMV-SCN2A-Variant-4-IRES-mScarlet

    Plasmid

    #209410

    Purpose

    Expression of wild-type neonatal isoform of human SCN2A.

    Depositor

    Al George

    Article

    Thompson et al bioRxiv. 2023 Feb 23:2023.02.23.529757.

    Insert

    SCN2A Variant 4, stabilized (SCN2A Human)
    

    Expression

    Mammalian

    Mutation

    IVS intron inserted after bp 4551 of the SCN2A OR…

    Promoter

    CMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PP7_mCherry

    Plasmid

    #61763

    Purpose

    Expresses PP7 coat protein fused to mCherry

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    PP7 coat protein fused to mCherry
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Ubc promoter

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PP7_mCherry

    Plasmid

    #61763

    Purpose

    Expresses PP7 coat protein fused to mCherry

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    PP7 coat protein fused to mCherry
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Ubc promoter

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

    Plasmid

    #208696

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

    Plasmid

    #208696

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid

    #92099

    Purpose

    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Expression

    Mammalian

    Promoter

    Tet-On 3G Bidirectional

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid

    #92099

    Purpose

    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Expression

    Mammalian

    Promoter

    Tet-On 3G Bidirectional

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFGC-pcoCas9

    Plasmid

    #52256

    Purpose

    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

    Depositor

    Jen Sheen

    Insert

    35SPPDK:pcoCas9:NOS cassette
    

    Use

    CRISPR; Plant expression

    Promoter

    constitutive 35SPPDK promoter

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFGC-pcoCas9

    Plasmid

    #52256

    Purpose

    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

    Depositor

    Jen Sheen

    Insert

    35SPPDK:pcoCas9:NOS cassette
    

    Use

    CRISPR; Plant expression

    Promoter

    constitutive 35SPPDK promoter

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV.hSyn.eGFP.WPRE.bGH

    Plasmid

    #105539

    Purpose

    AAV expression of EGFP from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV.hSyn.eGFP.WPRE.bGH

    Plasmid

    #105539

    Purpose

    AAV expression of EGFP from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYC-C

    Plasmid

    #207114

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYC-C

    Plasmid

    #207114

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge