We narrowed to 14,477 results for: cas9
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Plasmid#112065PurposeExpression and purification of NLSH2BCas9 for fungal gene editingDepositorInsertNLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
UseCRISPRTagsHis tagExpressionBacterialMutationCas9 was codon optimized for Aspergillus nigerPromoterpromoter in vectorAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 blast
Plasmid#104997PurposeThis lentiviral construct delivers hSpCas9 and blasticidin S resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_NPM1_G6
Plasmid#178091PurposeExpresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.DepositorInsertNPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRExpressionMammalianPromoterU6 promoterAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX461-ABE8e-nSpCas9
Plasmid#237460PurposeTransfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.DepositorInsert3xFlag_ABE8e_SpCas9(D10A)_6xNLS
UseCRISPRTags3xFLAGExpressionMammalianMutationD10APromoterchicken beta-actin promoter & U6Available SinceJan. 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
pX461-evoCDA1-nSpCas9
Plasmid#237461PurposeTransfection-based all-in-one base editor plasmid that expresses both cytosine base editor (evoCDA1-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.DepositorInsert3xFlag_evoCDA1_ssDBD_nickase SpCas9 _6xUGI
UseCRISPRTags3xFLAGExpressionMammalianMutationD10APromoterchicken beta-actin promoter & U6Available SinceJan. 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9-Dnmt3A/3L-V3
Plasmid#222500PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; R887E and E814ā¦PromoterEFSAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVT-dCas9-mCherry
Plasmid#174443Purposein vitro transcription template for dCas9-mCherryDepositorInsertCas9 Endonuclease Dead
UseIn vitro transcription templateTagsmCherryPromoterT7 SP6Available SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRS-AraC-dCas9
Plasmid#220195Purposemini-Tn7 dCas9 expression vector - AraC/pBAD promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterAraC/pBADAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Cas9-6xHis
Plasmid#62374PurposeExpression of Cas9-6xHis in bacterial cellsDepositorInsertCas9_6xHis
Tags6xHis tagExpressionBacterialPromoterT7Available SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
DHFR-SpCas9-DHFR
Plasmid#85447PurposeExpresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cellsDepositorInsertDHFR-SpCas9-DHFR
UseAAVTagsDestabilized domain (C-terminus version) of E.colā¦ExpressionMammalianPromoterCbhAvailable SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag(22aa)
Plasmid#215745PurposeExpresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkersDepositorInsertdCas9-10xSunTag
ExpressionMammalianPromoterchicken beta-actin promoterAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en1FnCas9
Plasmid#201947PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en1FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationE1369R on FnCas9PromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA_J23119-sgRNA
Plasmid#108550PurposeBacterial Target-AID vector with sgRNADepositorInsertsdCas9-PmCDA1
Streptococcus pyogenes sgRNA
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9PromoterJ23119 and lambda ORAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
TwinStrepSUMO-pvc10-Cas9
Plasmid#243755PurposeFor affinity purification of Pvc10 fused to Cas9DepositorInsertpvc10-Cas9
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDJ231 - Phsp Cas9
Plasmid#191382PurposeHeat shock inducible Cas9 expression in C. elegans.DepositorInsertPhsp-16.41 Cas9 gpd-2 tagRFP-t
UseCRISPRExpressionWormPromoterPhsp-16.41Available SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-Cas9
Plasmid#198275PurposeFor loading SpCas9 into PVCs; also contains PVC regulatory genesDepositorInsertPdp1_NTD-Cas9
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-GFP-VPR
Plasmid#181907PurposeFluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-RtaDepositorInsertdCas9-VPR
UseCRISPRTagsGFPExpressionMammalianPromoterCMVAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas9-recX
Plasmid#158715PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recXDepositorInsertcas9
UseCRISPRExpressionBacterialPromoterTetOAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-BsdCas9-W
Plasmid#67978PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termiā¦PromoterEF1aAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRTH
Plasmid#136925PurposeExpresses Cas9-NRTH in mammalian cellsDepositorInsertCas9-NRTH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only