We narrowed to 14,071 results for: cas9

1. PX458-3xHA-en31FnCas9

   Plasmid

   #201949

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en31FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   G1243T, E1369R, E1603H on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRZ-CAS9-mCherry

   Plasmid

   #80974

   Purpose

   Cas9, mCherry expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   Cas9
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTDH3-dCas9-Mxi1

   Plasmid

   #46921

   Purpose

   Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-Mxi1
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TDH3

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-ABE8e(V106W)

   Plasmid

   #198547

   Purpose

   Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458-3xHA-FnCas9

   Plasmid

   #130969

   Purpose

   3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   FnCas9
   

   Use

   CRISPR

   Tags

   3xHA-NLS and NLS-T2A-EGFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAS9-mCherry empty

   Plasmid

   #80975

   Purpose

   Cas9, mCherry, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74010

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5xUAS
   Cas9
   Cre
   U6 promoter
   

   Use

   CRISPR

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-2NLS-Cas9

   Plasmid

   #229773

   Purpose

   Expresses Cas9 driven by a CMV promoter

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

   Insert

   SpCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330 spCas9-mSA

   Plasmid

   #113096

   Purpose

   pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   CAS9-MSA
   

   Use

   Synthetic Biology

   Tags

   MSA (monomeric streptavidin

   Mutation

   none

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMDJ231 - Phsp Cas9

    Plasmid

    #191382

    Purpose

    Heat shock inducible Cas9 expression in C. elegans.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Phsp-16.41 Cas9 gpd-2 tagRFP-t
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    Phsp-16.41

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. modRNAc0-Cas9-2A-Puro

    Plasmid

    #172855

    Purpose

    IVT template plasmid for making modified Cas9Puro RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9-2A-Puro
    

    Use

    Ivt template plasmid

    Tags

    2A-Puro

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p-dCas9-LSD1-Hygro

    Plasmid

    #104406

    Purpose

    transient expression of dCas9-LSD1 fusion protein

    Depositor

    Stephan Beck

    Insert

    LSD1 (KDM1A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    IRATp970A0364D (Source Bioscience) has a P405H ch…

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEM-Cas9HF1-recA56

    Plasmid

    #89962

    Purpose

    Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Inserts

    Cas9HF1
    recA56
    

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet and proD

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiV2-EF1α-nCas9/RT

    Plasmid

    #210188

    Purpose

    Expression of nCas9/RT-P2A-Puro

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    nCas9/RT-P2A-Puro
    

    Use

    Lentiviral

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDe-CAS9-D10A

    Plasmid

    #61477

    Purpose

    based on pDe-CAS9, encodes Cas9 D10A nickase variant

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    catalytic Asp10 changed to Ala

    Promoter

    PcUbi4-2

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459V2.0-eSpCas9(1.1)

    Plasmid

    #108292

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE TRE dCas9

    Plasmid

    #50915

    Purpose

    Tet-regulatable dCas9 lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-dCas9-HDAC1-Hygro

    Plasmid

    #104409

    Purpose

    transient expression of dCas9-HDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330a dCas9-LSD1

    Plasmid

    #92362

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-LSD1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9(BB)-2A-Puro-SMCR8

    Plasmid

    #83440

    Purpose

    Expresses Cas9 and a gRNA targeting SMCR8

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human SMCR8 (SMCR8 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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