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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,125 results for: cas9

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Showing: 1061 - 1080 of 23125 results

  2. AIO-Puro

    Plasmid
    #74630
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Publication
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Puromycin resistant marker
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74630-plasmid-map-sequence-id-373032

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  4. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Publication
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  9. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Publication
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  15. pX-evoCas9

    Plasmid
    #107550
    Purpose
    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells
    Depositor
    Anna Cereseto
    Publication
    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    M495V/Y515N/K526E/R661Q
    Promoter
    CBh
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107550-plasmid-map-sequence-id-206015

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  18. M-SPn-VP64

    Plasmid
    #48674
    Purpose
    Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VP64, nuclease-null
    Use
    CRISPR
    Tags
    NLS and VP64
    Mutation
    nuclease-null
    Promoter
    CMV
    Available since
    Dec. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 1061 - 1080 of 23125 results