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166,542 results

Showing: 1081 - 1100 of 166542 results

  2. pAAV2/1

    Plasmid
    #112862
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap1
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Truncated P5
    Available Since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112862-plasmid-map-sequence-id-421935

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  3. T7-VEE-GFP

    Plasmid
    #58977
    Purpose
    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Insert
    EGFP
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    26S subgenomic promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58977-plasmid-map-sequence-id-419580

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  9. rtTA-N144

    Plasmid
    #66810
    Purpose
    2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance
    Depositor
    Andrew Yoo
    Article
    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi
    Insert
    Reverse tetracycline-controlled transactivator
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a
    Available Since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    66810-plasmid-map-sequence-id-418923

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  10. pRGEB32

    Plasmid
    #63142
    Purpose
    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Rice snoRNA U3 promoter for gRNA/PTG expression a…
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63142-plasmid-map-sequence-id-339931

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  12. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Tight TRE promoter
    Available Since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

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  13. pAAV-hSyn-mCherry

    Plasmid
    #114472
    Purpose
    mCherry Control
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available Since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114472-plasmid-map-sequence-id-220931

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  14. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106280-plasmid-map-sequence-id-354474

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  16. pGP-AAV-syn-iGABASnFR2-WPRE

    Plasmid
    #218874
    Purpose
    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)
    Depositor
    GENIE Project
    Article
    Kolb et al bioRxiv 2025.03.25.644953
    Has Service
    AAV1 and AAV5
    Insert
    iGABASnFR2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S99A F102Y F104Y L178S
    Promoter
    Synapsin
    Available Since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218874-plasmid-map-sequence-id-436585

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  18. MinLibCas9

    Pooled Library
    #164896
    Purpose
    Human genome-wide CRISPR-Cas9 gRNA library, optimised by mining existing large-scale screening datasets, which provides a >60% reduction in size while preserving key information content
    Depositor
    Mathew Garnett
    Article
    Gonçalves et al Genome Biol. 2021 Jan 21;22(1):40. doi:
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available Since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-hSyn-hM4D(Gi)-mCherry

    Plasmid
    #50475
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50475-plasmid-map-sequence-id-171072

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Showing: 1081 - 1100 of 166542 results