163,636 results

1. SB100X in pCAG globin pA

   Plasmid

   #127909

   Purpose

   Sleeping Beauty Transposase expression vector driven by the CAG promoter

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   Sleeping Beauty Transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. V5-TurboID-NES_pCDNA3

   Plasmid

   #107169

   Purpose

   expresses V5-tagged TurboID in the mammalian cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

   Plasmid

   #134868

   Purpose

   Expresses EGFP-tagged lysosomal marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lysosomal-associated membrane protein 1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-DIO-EGFP (AAV2)

   Viral Prep

   #50457-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   EGFP (Cre-dependent)

   Available since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-hSyn-mCherry

   Plasmid

   #114472

   Purpose

   mCherry Control

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-HA-Ubiquitin-K48R

   Plasmid

   #17604

   Purpose

   Mammalian expression of HA tagged ubiquitin K48R

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48R (expected to disrupt G76-K48 ubiquitin chain…

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. [#GS7-LV] DsRed GFP TEVp Zeo Switch - Recipient

   Plasmid

   #233507

   Purpose

   Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

   Insert

   DsRed loxP eGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-hM4D(Gi)-mCherry

   Plasmid

   #50475

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19

   Plasmid

   #50005

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lac

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PIEZO1-HaloTag

    Plasmid

    #207834

    Purpose

    PIEZO1 fusion for biochemistry and live imaging

    Depositor

    Charles Cox

    Article

    Zhou et al Science. 2023 Aug 18;381(6659):799-804.

    Insert

    PIEZO1 (PIEZO1 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL-7xHis-TEV-hexa

    Plasmid

    #222868

    Purpose

    Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

    Depositor

    Nikolaus Loening

    Article

    Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

    Insert

    TEV protease
    

    Use

    Tags

    7xHis and MBP

    Expression

    Bacterial

    Mutation

    C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3-N90-beta-catenin

    Plasmid

    #31785

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of aa 1-90- Constitutitvely Active

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. TK4-EGFP

    Plasmid

    #239047

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. L304-EGFP-Tubulin-WT

    Plasmid

    #64060

    Purpose

    EGFP fused at the N-terminus of tubulin; lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

    Insert

    tubulin
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-CamKIIa-jGCaMP8f-WPRE (AAV5)

    Viral Prep

    #176750-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from AAV-CamKIIa-jGCaMP8f-WPRE (#176750). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8f-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CamKIIalpha

    Tags

    None

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

17. pDx_mScarlet3

    Plasmid

    #189754

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP

    Plasmid

    #176015

    Purpose

    Mammalian expression of EGFP. Parton lab clone DFS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. ptfLC3

    Plasmid

    #21074

    Purpose

    Mammalian expression of rat LC3 fused to mRFP and EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV9)

    Viral Prep

    #105545-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    HI

    Tags

    EGFP

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only