We narrowed to 19,528 results for: MUT

Showing: 11741 - 11760 of 19528 results

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pEB1-sfGFP

Plasmid

#103983

Purpose

Plasmid encoding sfGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

sfGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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EC2_7_dCas9_HC_sgRNA

Plasmid

#163711

Purpose

High copy plasmid with dCas9 and sgRNA expression cassettes

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ155

Plasmid

#69306

Purpose

Gateway entry vector with AteCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9D10A (Arabidopsis codon optimized)

Use

CRISPR

Mutation

D10A mutation; NLS fusion

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AksgRNA

Plasmid

#121955

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b single chimeric gRNA

Expression

Mammalian

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pALS3-Ma-SUMO-sfGFP-WT

Plasmid

#212122

Purpose

Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

Depositor

Ryan Mehl

Inserts

SUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)

Tags

His6 and SUMO

Expression

Bacterial

Promoter

araC and lpp

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a H759A

Plasmid

#113518

Purpose

Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Mutation

H759A

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA

Plasmid

#121954

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-PICK1-rescue

Plasmid

#72573

Purpose

Expresses myc-PICK1 shRNA resistant cDNA

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1 Rat)

Tags

Myc

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARα-T391A

Plasmid

#119920

Purpose

Mammalian expression of the tAKARα sensor with T391A mutation

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα-T391A

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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PKCalpha.K/N.FLAG

Plasmid

#10806

Depositor

Alex Toker

Article

Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

Insert

PKC alpha (PRKCA Bovine)

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K368N (Lys 368 to Asn)

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pUC19-U6-AasgRNA3.8

Plasmid

#121958

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA, short version

Expression

Mammalian

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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HOXA9-MSCV-full-GFP

Plasmid

#20977

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

Insert

HOXA9 (Hoxa9 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Full length cDNA with ~1.1kb 3'UTR with 2 pu…

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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p413TEF-Sir2-H364Y

Plasmid

#51743

Purpose

overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

Sir2 (SIR2 Budding Yeast)

Expression

Yeast

Mutation

H364Y

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pReporter_2

Plasmid

#60563

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_2

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pUG-248

Plasmid

#104709

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

PUB22 C13A (PUB22 Mustard Weed)

Use

Synthetic Biology

Mutation

C13A, BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-21

Plasmid

#104707

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

PUB22 WT (PUB22 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Syn:eGFP-CaM-uTEV1Δ(220-242)

Plasmid

#135463

Purpose

Neuronal expression of uFLARE protease component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

eGFP-CaM-uTEV1Δ

Use

AAV

Tags

eGFP, V5

Expression

Mammalian

Mutation

S219V mutation improves stability and S153N impro…

Promoter

Synapsin

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-YOD1 C160S

Plasmid

#49785

Purpose

Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Tags

Flag-tag

Mutation

C160S

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-FRB-LplA(W37V)

Plasmid

#35608

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FRB-LplA(W37V)

Tags

FLAG (internal fusion), HA, and His Tag

Expression

Mammalian

Mutation

LplA has Trp37 to Val mutation

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pICSL50015

Plasmid

#50318

Purpose

Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mNEON (Branchistoma lanceolatum)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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