Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

167,318 results

Showing: 1741 - 1770 of 167318 results

  9. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212962
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_non-targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212962-plasmid-map-sequence-id-422485

    Enlarge

  11. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212963
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_GFP targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212963-plasmid-map-sequence-id-422499

    Enlarge

  12. pCW57-GFP-2A-MCS

    Plasmid
    #71783
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Available Since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    71783-plasmid-map-sequence-id-197192

    Enlarge

  13. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

    Plasmid
    #234952
    Purpose
    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234952-plasmid-map-sequence-id-467733

    Enlarge

  15. RHO3

    Bacterial Strain
    #124700
    Purpose
    E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.
    Depositor
    Herbert Schweizer
    Article
    Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):
    Bacterial Resistance
    Dap (400 µg/ml)
    Available Since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV-hSyn-hM3D(Gq)-mCherry (AAV2)

    Viral Prep
    #50474-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. pCAG-T7pol

    Plasmid
    #59926
    Purpose
    Expression vector for T7 RNA polymerase gene
    Depositor
    Ian Wickersham
    Insert
    T7 RNA polymerase
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    59926-plasmid-map-sequence-id-418961

    Enlarge

  27. pAAV-Syn-ChrimsonR-tdT (AAV9)

    Viral Prep
    #59171-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  28. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67638-plasmid-map-sequence-id-322882

    Enlarge

  30. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV1)

    Viral Prep
    #162378-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 1741 - 1770 of 167318 results