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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 57,040 results for: REP

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Showing: 1161 - 1180 of 57040 results

  3. lucMAPT-GenRep

    Plasmid
    #214674
    Purpose
    MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9 and Exon 11, with a BamHI EcoRI c…
    Promoter
    CMV
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214674-plasmid-map-sequence-id-424937

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  4. TMC1-TwinStrepII

    Plasmid
    #83267
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    TMC1 (Tmc1 Mouse)
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83267-plasmid-map-sequence-id-162234

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  8. pAB1923 REPAIR.t1-S

    Plasmid
    #176321
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q/E620G/Q696L, only the deaminase domain (aa …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176321-plasmid-map-sequence-id-345288

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  12. pET28-3'TwStrep

    Plasmid
    #208053
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208053-plasmid-map-sequence-id-415923

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  17. pET28-5'TwStrep

    Plasmid
    #208620
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208620-plasmid-map-sequence-id-415919

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  18. CACNA2D1-TwinStrepII

    Plasmid
    #83192
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    CACNA2D1 (Cacna2d1 Rat)
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83192-plasmid-map-sequence-id-163487

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  19. pCRISPReporter-mCherry

    Plasmid
    #65008
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    User-defined
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65008-plasmid-map-sequence-id-109895

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Showing: 1161 - 1180 of 57040 results