174,897 results
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Plasmid#207834PurposePIEZO1 fusion for biochemistry and live imagingDepositorAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only
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pAAV-hSyn-hM4D(Gi)-mCherry (AAV8)
Viral Prep#50475-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherryAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC1-SypHer3s-dmito
Plasmid#108119PurposeMitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.DepositorInsertSypHer3s-dmito
Tagsratiometric YFPExpressionMammalianPromoterCMVAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRi-v2, Membrane Proteins (h6), top 5 sgRNAs/gene
Pooled Library#83976PurposeHuman CRISPRi Pooled Library targeting membrane proteinsDepositorAvailable SinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJSK664
Plasmid#205130PurposeExpression of GFP-2xPX (from SnxA) in mammalian cellsDepositorInserttandem 2xPX domain of SnxA
TagsEGFPExpressionMammalianAvailable SinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-iGABASnFR2-WPRE (AAV1)
Viral Prep#218874-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-iGABASnFR2-WPRE (#218874). In addition to the viral particles, you will also receive purified pGP-AAV-syn-iGABASnFR2-WPRE plasmid DNA. Syn-driven expression of the improved GABA sensor iGABASnFR2 (positive change in fluorescence). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-BSD
Plasmid#174821PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
TagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd3
Plasmid#98298PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP‐RNASEH1
Plasmid#108699PurposeFor expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cellsDepositorAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1+SKL
Plasmid#53450Purposeexpresses GFP targeted for localization in peroxisomesDepositorInsertSKL amino acids
TagsSKLExpressionMammalianPromoterCMVAvailable SinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-hPIKfyve
Plasmid#121148PurposeExpresses GFP-tagged human PIKfyve in mammalian cells.DepositorInsert1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
TagsGFPExpressionMammalianPromoterCMVAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
RNA-Protein Landing Pad
Plasmid#226162PurposeUsed to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expressionDepositorInsertsmTagBFP2-P2A-iCasp9
Reverse tetracycline transactivator 3
Hygromycin-IRES-EGFP
UseSynthetic BiologyTagsHAExpressionMammalianAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)
Plasmid#163059Purposemito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)DepositorInsertmito-roGFP2-Orp1
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Olig001
Plasmid#170716PurposeOlig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNSDepositorInsertOlig001 capsid gene / rep 2
UseAAVAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-gfap
Plasmid#75024PurposeCan be used to drive expression in zebrafish astrocytes.DepositorInsertglial fibrillary acidic protein promoter (gfap Zebrafish)
Use5' entry vector for multisite gateway three …PromotergfapAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUGW-pHRed
Plasmid#65742PurposeLentiviral vector for pHRed expression in mammalian cells using the hUbiC promoterDepositorInsertpHRed
UseLentiviralExpressionMammalianPromoterhUbiCAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1
Plasmid#193356PurposeVEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCsDepositorUseVenezuelan equine encephalitis (vee) virus rna re…ExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…Available SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
His6-pAG-RTase
Plasmid#214932PurposeExpress fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systemsDepositorInsertfusion protein of protein A/G and MMLV reverse transcriptase (25-497)
TagsHis6ExpressionBacterialMutationMMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…PromoterT7 promoterAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
MoClo Yeast Secretion and Display (YSD) Toolkit
Plasmid Kit#1000000240PurposeCollection of plasmids to optimize secretion and cell surface display of proteins of interest in yeast.DepositorApplicationCloning and Synthetic BiologyVector TypeYeast ExpressionCloning TypeGolden Gate (MoClo)Available SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)
Viral Prep#154867-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available SinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only