We narrowed to 29,684 results for: REP

1. pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115267

   Purpose

   Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   LbuCas13a
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SOX9-T2A-H2B-EGFP repair template

   Plasmid

   #167972

   Purpose

   SOX9 reporter repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   SRY-Box Transcription Factor 9 (SOX9 Human)
   

   Use

   CRISPR; Donor template

   Tags

   H2B-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

   Plasmid

   #174424

   Purpose

   Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(Thrombin)EGFP(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

   Plasmid

   #113884

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   pHR-CMV-TetO2

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-AU1

   Plasmid

   #162091

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-ProtC-AU1
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

   Plasmid

   #84471

   Purpose

   pAAV-RAM-ChR2

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   Channelrhodopsin 2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mutation

   H134R

   Promoter

   TRE

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115270

   Purpose

   Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   TtCsm6
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. delta PE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52380

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted -1620 - -1011 upstream from OCT4 ATG

   Promoter

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. delta DE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52381

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted -3194 - -1768 upstream from OCT4 ATG

   Promoter

   OCT4

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mouse deltaPE Oct4-GFP transgenic reporter

    Plasmid

    #52382

    Purpose

    BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP pA
    Oct4 (Pou5f1 Mouse)
    PGK-gb2-Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted -300 - -1300bp fragment upstream from Oct…

    Promoter

    Oct4 and PGK+gb2

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNL(CMV)Rep78-III/CAG/WPREΔU3

    Plasmid

    #190727

    Purpose

    Expression of AAV2 Rep78-III in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Li et al Hum Gene Ther Methods. 2018 Jun;29(3):13

    Insert

    AAV2 Rep78-III
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

    Plasmid

    #172620

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-CMV-TetO2_3C-mVenus-Twin-Strep

    Plasmid

    #113891

    Purpose

    Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-mVenus-Twin-Strep

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mouse Oct4-GFP GOF18 transgenic reporter

    Plasmid

    #60527

    Purpose

    BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    GFPpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Oct4

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)

    Plasmid

    #44094

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    2xStrep tag, 6xGly linker, ADH1 transcription ter…

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTF279 (pFA6a-TEV-6xGly-2xStrep-HphMX)

    Plasmid

    #44082

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    2xStrep tag, 6xGly linker, ADH1 transcription ter…

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiUBC CASP1[CARD]-mSG2 (inflammasome reporter)

    Plasmid

    #228044

    Purpose

    Lentiviral stable Inflammasome speck reporter, designed from PMC9623368.

    Depositor

    Wade Harper

    Insert

    CASP1[CARD]-mSG2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

    Plasmid

    #172627

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223 CASP1[CARD]-mSG2 (inflammasome reporter)

    Plasmid

    #228043

    Purpose

    DONR vector for inflammasome speck reporter, designed from PMC9623368.

    Depositor

    Wade Harper

    Insert

    CASP1[CARD]-mSG2
    

    Use

    Gateway donr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Enhanced Repressor Domain for TAL Effector (SID4X)

    Plasmid

    #43882

    Depositor

    Feng Zhang

    Article

    Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

    Insert

    SID4X Domain
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only