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We narrowed to 18,344 results for: multi

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Showing: 1181 - 1200 of 18344 results

  2. pHL-EF1a-SphcCas9-iP-A

    Plasmid
    #60599
    Purpose
    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
    Depositor
    Akitsu Hotta
    Publication
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR Cas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon usage optimized for human usage
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    60599-plasmid-map-sequence-id-97109

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  3. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid
    #174665
    Purpose
    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter
    Depositor
    Eli Bar
    Inserts
    LUC2
    mCherry:T2A:Hygro
    Use
    Lentiviral and Luciferase
    Tags
    mCherry and hygromycin dual reporter gene linked …
    Expression
    Mammalian
    Promoter
    Human cytomegalovirus immediate early enhancer/pr…
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    174665-plasmid-map-sequence-id-344886

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  7. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    86612-plasmid-map-sequence-id-168849

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  13. delta PE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid
    #52380
    Purpose
    BAC recombineering generated construct, used as a reporter for OCT4 expression
    Depositor
    Jacob Hanna
    Publication
    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GFP-2A-puro
    OCT4 (POU5F1 Human)
    Expression
    Mammalian
    Mutation
    deleted -1620 - -1011 upstream from OCT4 ATG
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    52380-plasmid-map-sequence-id-82023

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  14. pDule-tfmF A65V S158A

    Plasmid
    #85484
    Purpose
    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
    Depositor
    Ryan Mehl
    Publication
    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.
    Insert
    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32V F108W Q109M I159M with 65Val and 158Ala
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    85484-plasmid-map-sequence-id-169864

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  17. pENTR11 CA NFAT2

    Plasmid
    #11793
    Depositor
    Anjana Rao
    Insert
    NFAT2 (Nfatc1 Mouse)
    Expression
    Bacterial
    Mutation
    Constitutively active: multiple serine to alanine…
    Available since
    Aug. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles

  18. pcDNA3-ExRai-AktAR

    Plasmid
    #118462
    Purpose
    cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AktAR
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    118462-plasmid-map-sequence-id-230151

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  19. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid
    #159149
    Purpose
    expresses His-tagged HIV reverse transcriptase and untagged HIV protease
    Depositor
    Andrea Pauli
    Publication
    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d
    Inserts
    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    Tags
    His
    Expression
    Bacterial
    Mutation
    HIV-1 group M/subtype B – BH10 strain
    Available since
    Sept. 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    159149-plasmid-map-sequence-id-310074

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  20. pVITRO1-M80-F2-IgG1/κ

    Plasmid
    #50383
    Purpose
    Expresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chain
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    human Gamma 1 heavy chain expression cassette with unknown specificity
    human Kappa light chain expression cassette with unknown specificity
    Expression
    Mammalian
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    Jan. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    50383-plasmid-map-sequence-id-75289

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Showing: 1181 - 1200 of 18344 results