We narrowed to 31,602 results for: REP

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SOX9-T2A-H2B-EGFP repair template

Plasmid

#167972

Purpose

SOX9 reporter repair template

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

SRY-Box Transcription Factor 9 (SOX9 Human)

Use

CRISPR; Donor template

Tags

H2B-EGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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delta PE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52380

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Expression

Mammalian

Mutation

deleted -1620 - -1011 upstream from OCT4 ATG

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pFl Strep-Sumo-TEV human OAS1

Plasmid

#130895

Purpose

Human oligoadenylate synthase 1 (OAS1) is used to label terminal ADP-riobos

Depositor

Leemor Joshua-Tor

Article

Ando et al Mol Cell. 2019 Feb 21;73(4):845-856.e5.

Insert

oligoadenylate synthase 1 (OAS1 Human)

Tags

Strep_Sumo

Expression

Insect

Promoter

ph

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)

Plasmid

#44070

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

2xStrep tag, 6xGly linker, ADH1 transcription ter…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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delta DE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52381

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Expression

Mammalian

Mutation

deleted -3194 - -1768 upstream from OCT4 ATG

Promoter

OCT4

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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mouse deltaPE Oct4-GFP transgenic reporter

Plasmid

#52382

Purpose

BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP pA
Oct4 (Pou5f1 Mouse)
PGK-gb2-Neo

Expression

Mammalian

Mutation

deleted -300 - -1300bp fragment upstream from Oct…

Promoter

Oct4 and PGK+gb2

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115270

Purpose

Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

TtCsm6

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-Enh eGFP Reporter-muKC2-X11

Plasmid

#59235

Purpose

Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

Depositor

Benjamin Yu

Insert

Krt7-ex2

Use

Lentiviral

Promoter

Mu Hsp68 minimal promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pHR-CMV-TetO2_3C-mVenus-Twin-Strep

Plasmid

#113891

Purpose

Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-mVenus-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-AU1

Plasmid

#162111

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to StrepTagII-ProtC-AU1

Use

Lentiviral

Tags

StrepTagII-ProtC-AU1

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-FLAG

Plasmid

#162090

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to StrepTagII-ProtC-FLAG

Use

Lentiviral

Tags

StrepTagII-ProtC-FLAG

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-AU1

Plasmid

#162082

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-StrepTagII-AU1

Use

Lentiviral

Tags

VSVg-StrepTagII-AU1

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

Plasmid

#174424

Purpose

Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

6xHis(Thrombin)EGFP(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28-Strep-OPA1(253-960) (SB263)

Plasmid

#227612

Purpose

Inducible expression of Strep-tagged Opa1(253-960) in E.coli

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Bacterial

Mutation

contains aa 253-960 only

Promoter

T7

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-AU1

Plasmid

#162102

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to VSVg-StrepTagII-AU1

Use

Lentiviral

Tags

VSVg-StrepTagII-AU1

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-FLAG

Plasmid

#162110

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to StrepTagII-ProtC-FLAG

Use

Lentiviral

Tags

StrepTagII-ProtC-FLAG

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-StrepTagII-FLAG-AU1

Plasmid

#162094

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to StrepTagII-FLAG-AU1

Use

Lentiviral

Tags

StrepTagII-FLAG-AU1

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-FLAG

Plasmid

#162081

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-StrepTagII-FLAG

Use

Lentiviral

Tags

VSVg-StrepTagII-FLAG

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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Enhanced Repressor Domain for TAL Effector (SID4X)

Plasmid

#43882

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

SID4X Domain

Available Since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
4A3.PBAD-repL(AT2).J23115-gfp

Plasmid

#197873

Purpose

Arabinose induced expression of repL(AT2) gene, which triggers DNA replication in trans, weak level of constitutive GFP expression

Depositor

Baojun Wang

Article

Huan et al Front Microbiol. 2023 Feb 9;14:1095671.

Insert

part kilA/repL(AT2)

Expression

Bacterial

Promoter

PBAD

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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