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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,252 results for: Sele

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Showing: 2561 - 2600 of 23252 results

  2. 4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172228
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells
    Depositor
    Joshua Rosenthal
    Article
    Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Tags
    6xHis and Flag
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172228-plasmid-map-sequence-id-339581

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  4. LLP618_αGCN4-KRAB-CO

    Plasmid
    #211782
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    aGCN4-mCherry
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect mCherr…
    Promoter
    pEF1a and pSV40
    Available Since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    211782-plasmid-map-sequence-id-417156

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  9. SSTR2-DuET

    Plasmid
    #213377
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR2 (SSTR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213377-plasmid-map-sequence-id-430733

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  16. pYZ145

    Plasmid
    #98405
    Purpose
    Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.
    Depositor
    Jef Boeke
    Article
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Inserts
    upstream homologous region to delete leu1 in genome by recombination (apc2 Fission Yeast)
    downstream homologous region to delete leu1 in genome by recombination (top2 Fission Yeast)
    Use
    CRISPR and Synthetic Biology
    Available Since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98405-plasmid-map-sequence-id-198862

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  18. pYZ149

    Plasmid
    #98407
    Purpose
    Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.
    Depositor
    Jef Boeke
    Article
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Inserts
    upstream homologous region to delete his3 in genome by recombination (alg2 Fission Yeast)
    downstream homologous region to delete his3 in genome by recombination (cog8 Fission Yeast)
    Use
    CRISPR and Synthetic Biology
    Available Since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98407-plasmid-map-sequence-id-198894

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  20. pGS1021

    Plasmid
    #59575
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Promoter
    None
    Available Since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    59575-plasmid-map-sequence-id-92981

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  24. pMpGWB122

    Plasmid
    #68576
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available Since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    68576-plasmid-map-sequence-id-121831

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  32. pYPQ166-iSpyMac

    Plasmid
    #149553
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    iSpyMacCas9
    Use
    CRISPR; Gateway compatible zispymaccas9 entry clo…
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    R221K, N394K, Protospacer interacting domain form…
    Available Since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149553-plasmid-map-sequence-id-306139

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Showing: 2561 - 2600 of 23252 results