We narrowed to 10,491 results for: aga

1. BC-A1-013

   Plasmid

   #78701

   Purpose

   A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGK415-­phlTA2-­1E

   Plasmid

   #165970

   Purpose

   Expresses phlTA(2-­1E) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   phlF
   

   Use

   Synthetic Biology

   Tags

   Nuclear localization signal and three copies of V…

   Expression

   Yeast

   Mutation

   P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

   Promoter

   pPGK1 mutant

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET15b+PCNA1 WT

   Plasmid

   #66139

   Purpose

   Expresses the wild type of Sulfolobus solfataricus PCNA1

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Nano-lantern-Actin/pEGFP-C1

   Plasmid

   #51972

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   Actin

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pAID1.2-CMV-NmScarlet-mAID

   Plasmid

   #140618

   Purpose

   Expression of OsTIR1 and mScarlet-mAID protein in DT40 cells under the control of CMV promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Nano-lantern-peroxisome/pcDNA3

   Plasmid

   #51974

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to peroxisomes

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   SKL

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

7. Nano-lantern(Ca2+)_300/pRSETB

   Plasmid

   #51977

   Purpose

   bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM_E104Q-3GS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. BC-A1-005

   Plasmid

   #78692

   Purpose

   A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CT-Nano-lantern(ATP1)/pRSETB

   Plasmid

   #51990

   Purpose

   bacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_ATP1
   

   Use

   Tags

   6xHis and chloroplast transit peptid

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Nano-lantern(cAMP-3.3)/pRSETB

    Plasmid

    #53592

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

11. Nano-lantern(Ca2+)_11/pRSETB

    Plasmid

    #51981

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM-4GS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pTH1-SaSrtA-KA

    Plasmid

    #64981

    Purpose

    Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108A

    Promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CT-Nano-lantern(ATP1)/pENTR1A

    Plasmid

    #51991

    Purpose

    Gateway entry vector for Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Entry vector

    Tags

    chloroplast transit peptid

    Expression

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. BC-A1-014

    Plasmid

    #78702

    Purpose

    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPIC-mouseKir3.2-mC-S

    Plasmid

    #140745

    Purpose

    Expression of mouse Kir3.2 fused to mCherry and Streptag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

    Insert

    Kir3.2 (Kcnj6 Mouse)
    

    Use

    Tags

    mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pattB-3xFlag-Loqs-PD

    Plasmid

    #41110

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    Loquacious-PD (loqs Fly)
    

    Use

    Tags

    3xFlag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Nano-lantern(ATP1)/pRSETB

    Plasmid

    #51988

    Purpose

    bacterial expression of a Nano-lantern-based luminescent ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Nano-lantern(Ca2+)_63/pcDNA3

    Plasmid

    #51986

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-4GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pSP571

    Plasmid

    #139498

    Purpose

    CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pGPD Cas9 / sgRNA (STL162)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pGPD

    Available since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UAS::PTPRDb-DN-GFP

    Plasmid

    #37112

    Depositor

    Alvaro Sagasti

    Article

    Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

    Insert

    UAS::PTPRDb-DN-GFP (ptprdb Zebrafish)
    

    Use

    Tol2 gateway destination vector

    Tags

    EGFP

    Expression

    Mutation

    deleted cytodomain, including phosphatase domains…

    Promoter

    UAS

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge