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163,403 results

Showing: 2601 - 2640 of 163403 results

  5. pCeru-N1 cyclin A (1557)

    Plasmid
    #61842
    Purpose
    mammalian expression of cyclin A with cerulean fusion
    Depositor
    Jonathon Pines
    Insert
    cyclin A (CCNA2 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pOpen-PstIM

    Plasmid
    #165536
    Purpose
    Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    M.PstI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165536-plasmid-map-sequence-id-339126

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  10. pRDB_347

    Plasmid
    #231114
    Purpose
    CBE activity-based selection cloning vector
    Depositor
    David Root, John Doench
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231114-plasmid-map-sequence-id-458384

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  11. pRD405

    Plasmid
    #163110
    Purpose
    Expression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mScarlet-I_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163110-plasmid-map-sequence-id-321851

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  20. pRD406

    Plasmid
    #163111
    Purpose
    Expression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mNeonGreen_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163111-plasmid-map-sequence-id-321832

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  21. pRD399

    Plasmid
    #163106
    Purpose
    Expression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mEGFP_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163106-plasmid-map-sequence-id-321847

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  22. FRIG-myc-Sema4D-CD4

    Plasmid
    #51606
    Purpose
    expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone
    Depositor
    Suzanne Paradis
    Article
    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do
    Insert
    Sema4D/CD4-FLAG (Sema4d Mouse, Human)
    Use
    Lentiviral
    Tags
    FLAG and myc
    Expression
    Mammalian
    Mutation
    Sema4D amino acids 660-861 were replaced with CD4…
    Promoter
    RSV
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  23. NG-ABE9

    Plasmid
    #240625
    Purpose
    Adenine base editor with narrowed editing window, recognizing NG PAM
    Depositor
    Tingting Jiang
    Insert
    TadA8e (N108Q/L145T)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    TadA8e (N108Q/L145T), Cas9 (D10A, L1111R, D1135V,…
    Promoter
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240625-plasmid-map-sequence-id-479197

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  24. NanoBRET Assay Vector, HaloTag-APC

    Plasmid
    #237145
    Purpose
    Express HaloTag(R)-APC Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Early Access Program
    Has Service
    DNA
    Insert
    APC (APC Human)
    Use
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237145-plasmid-map-sequence-id-473380

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  26. pAL005

    Plasmid
    #233865
    Purpose
    MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
    Depositor
    Jeff Hasty
    Article
    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102
    Insert
    pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    233865-plasmid-map-sequence-id-468718

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  28. TK4-NLS-EGFP

    Plasmid
    #239046
    Purpose
    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation
    Depositor
    Marius Wernig
    Inserts
    EGFP
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2 and c-myc-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1a
    Available since
    May 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    239046-plasmid-map-sequence-id-473412

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  30. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Synthetic, Human)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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  32. AiP13041 - pAAV-AiE0774m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3041)

    Plasmid
    #224026
    Purpose
    AiE0774m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L6_IT_Car3 neurons (note CLA neurons)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    224026-plasmid-map-sequence-id-449736

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  33. pD649-HAsp-SEMA5A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157008
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SEMA5A (SEMA5A Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157008-plasmid-map-sequence-id-303125

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  34. pSilencer2.1-U6-3JB1F+6

    Plasmid
    #190835
    Purpose
    For mammalian expression of 3JB1F+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F_T1+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190835-plasmid-map-sequence-id-379214

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  35. pAAV-CaMKIIa-hM4D(Gi)-mCherry (AAV8)

    Viral Prep
    #50477-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-hM4D(Gi)-mCherry (#50477). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM4D(Gi)-mCherry plasmid DNA. CaMKIIa-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    mCherry
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2601 - 2640 of 163403 results