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Plasmid#221228PurposeExpress HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase.DepositorInsertTadA8e-PcrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA PSPC1
Plasmid#101764PurposeExpresses PSPC1 in mammalian cellsDepositorInsertparaspeckle component 1 (PSPC1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
p52sheLL
Plasmid#46950PurposeHPV52 L1 and L2 Bicistronic, too large to self-packageDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (AAV8)
Viral Prep#154868-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-DTAG-NTERM-GFP
Plasmid#185775PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterPGKAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p31sheLL
Plasmid#37322DepositorInsertHPV31L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern
Plasmid#197928PurposeMonomer expression vectorDepositorInsertmGreenLantern
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6f.WPRE.SV40
Plasmid#100837PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CR029
Plasmid#234041Purposeexpresses evoCDA1 BE4max base editor in a human T cell optimized lentiviral transfer plasmidDepositorInsertevoCDA1-Cas9(D10A)-P2A-blast
UseLentiviralTagsblastExpressionMutationPromoterAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CR102
Plasmid#234043Purposeexpresses ABE8e(V106W) - SpG Cas9 base editor in a human T cell optimized lentiviral transfer plasmidDepositorInsertTadA8e(V106W)-SpG-Cas9(D10A)-P2A-blast
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO EYFP (AAV5)
Viral Prep#55641-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Flp-dependent)Available sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28c-F30-2xdBroccoli
Plasmid#66843PurposeExpresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLS-mP
Plasmid#81225PurposeLentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterDerived from pGL4.23Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FMRP_P451S
Plasmid#198693PurposeExpression of human FMRP protein in E. coliDepositorInsertFMR1 (FMR1 Human)
UseTagsMBPExpressionBacterialMutationchanged proline 451 to serinePromoterT7Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mEGFP-TSG101
Plasmid#116925Purposeexpresses mEGFP-TSG101 in mammalian cellsDepositorInsertTSG101 (TSG101 Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEff
Plasmid#97423PurposeTransient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES-jRCaMP1b.WPRE.SV40
Plasmid#100851PurposeAAV expression of jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV1 and AAV9InsertjRCaMP1b
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
PGK-SMASH-CTERM-GFP
Plasmid#185776PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, SMAShExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-mCherry-53BP1
Plasmid#127658PurposeOver-express mCherry-53BP1 fragment to monitor 53BP1 foci.DepositorInsert53BP1 (TP53BP1 Human)
UseLentiviralTagsmCherryExpressionMutationNucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…PromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-BFP
Plasmid#188775PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9-mTagBFP2
UseLentiviralTagsHA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA707
Plasmid#186238PurposeKanR-BBR1 entry vector for HR donorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RIPK3
Plasmid#78804PurposeTo overexpress RIPK3 in Mammalian CellsDepositorInsertRIPK3 (RIPK3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT3B1
Plasmid#35522PurposeMammalian expression of human DNMT3B1 with myc tagDepositorInsertDNMT3B1 (DNMT3B Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
SIRT7 Flag
Plasmid#13818PurposeMammalian expression of human SIRT7 with flag tagDepositorInsertSIRT7 (SIRT7 Human)
UseTagsflagExpressionMammalianMutationMissing exon 2PromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
SBFP2-anti-AlfaTag nanobody
Plasmid#171826PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertSBFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-Alb-mNG
Plasmid#183466PurposeTo produce AAV with the CBh promoter to express Alb(mouse)-mNeonGreen fusion proteinDepositorInsertAlb (Alb Mouse)
UseAAVTagsmNeonGreenExpressionMutationPromoterAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-Flpe:GFP
Plasmid#13788PurposeMammalian expression of enhanced form of Flp recombinase fused to GFPDepositorInsertFlpe-GFP fusion protein
UseFlp/frtTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV
Plasmid#16403PurposeShuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Fab_8D3_2_H-H6
Plasmid#176075PurposeExpress His6 tagged heavy chain of Fab_8D3_2 in mammalian cellsDepositorInsertHis6 tagged heavy chain of Fab_8D3_2
UseTagsHis tagExpressionMammalianMutationPromoterCAG promoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Fab_8D3_2_L
Plasmid#176076PurposeExpress light chain of Fab_8D3_2 in mammalian cellsDepositorInsertLight chain of Fab_8D3_2
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-GFP
Plasmid#181906PurposeFluorescently tagged dead Cas9DepositorInsertdCas9
UseCRISPRTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mGreenLantern:ALFA
Plasmid#219644PurposePermits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest methodDepositorInsertmGreenLantern:ALFA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2
Plasmid#75112Purposelenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-VPR
Plasmid#191269PurposepiggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFPDepositorInsertsVPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDG461
Plasmid#100902PurposeSpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.DepositorInsertshumanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-EGFPExpressionMammalianMutationD10A mutant converts to NickasePromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pR26
Plasmid#127372PurposeAllows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMutationPromotertight TRE promoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCI_FLAG-GFP(Y39TAG)-6His
Plasmid#223507PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
UseTags6xHis and FLAGExpressionMammalianMutationY39TAGPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP_PrA/G-H6
Plasmid#176077PurposeExpress His6 tagged MBP_PrA/G in Escherichia coliDepositorInsertMBP PrA/G-H6
UseTagsHis tagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only