We narrowed to 107,934 results for: rest
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Plasmid#99866PurposeEncodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0DepositorInsertUniSAM-mCherry + U6-gRNA2.0
UseCRISPR and Synthetic Biology; Dcas9-sam activationExpressionMammalianMutationBbsI sites in CDS were ablated by consensus mutat…PromoterEF1aAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
YAP/TAZ tdTomato TEAD Binding Reporter
Plasmid#228554PurposeEncodes a tdTomato fluorescent protein gene driven by a TEAD binding synthetic promoter. Used to track YAP/TAZ signaling pathway activity.DepositorInserttdTomato
UseLentiviralExpressionMammalianPromoterGTIIC TEAD-Binding Responsive ElementAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT/Caggs-NRASV12
Plasmid#20205DepositorAvailable SinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-Cezanne (OTU, aa 53-446)
Plasmid#61581PurposeExpresses human Cezanne (OTU domain) in E. coli.DepositorInsertCezanne (OTUD7B Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-52 and aa 447-843.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-NBK-1
Plasmid#207639PurposeA plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.DepositorInsertPyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
ExpressionBacterialMutationThe evolved synthetase NBK-1 contains mutations Y…PromoteraraBADAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
cBEST4
Plasmid#234660PurposeExpresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertionDepositorInsertsspCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP3 and tcp830Available SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
elavl3:hArr-TEV;he1.1:YFP
Plasmid#218855PurposeD. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.DepositorInserthArr
Tagshuman Arrestin-Tobbaco Etch Virus protease fusion…ExpressionBacterialAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-SDHB-Halo7Tag
Plasmid#108973PurposeSelflabeling tag at succinate dehydrogenase complex subunit B (SDHB)DepositorInsertsuccinate dehydrogenase complex iron sulfur subunit B (SDHB Human)
TagsHalo7-tagExpressionMammalianPromoterCMVAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCALPs-HA-NFAT1 (4-460)-GFP
Plasmid#88879PurposeLentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFPDepositorInsertHA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
UseLentiviralTagsGFP fusion protein and HA tagExpressionMammalianMutationamino acids 4-460 only; L78P & L287P *see bel…PromoterSFFV (spleen focus forming virus)Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-ATG5
Plasmid#99573PurposeExpresses Cas9 with sgRNA targeting Exon 7 of ATG5DepositorAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW-tTRE-scFv-SunTag
Plasmid#193138PurposeExpresses scFv intracellular antibody component of the SunTag system under Dox regulationDepositorInsertscFv-SunTag
UseLentiviralExpressionMammalianPromotertight TRE promoterAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUB1 (full-length, aa 1-271)
Plasmid#61420PurposeExpresses human OTUB1 (full-length) in E. coli.DepositorInsertOTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)
TagsHis6-GST-3CExpressionBacterialPromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3HD1RLuc2
Plasmid#236276PurposeMammalian expression of human dopamine D1 receptor; N-terminal HA tag, C-terminal Rluc8DepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE mNeonGreen-Core (HBc from HBV) IRES puro
Plasmid#122202PurposePlasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.DepositorInsertsHBc from HBV AYW
mNeonGreen
TagsGGSGGSGGS linkerExpressionMammalianAvailable SinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_Sec61B-V5-APEX2
Plasmid#83411PurposeExpressed C-term tagged APEX2 on Sec61B in mammalian cellDepositorInsertSec61B-V5-APEX2 (SEC61B Synthetic, Human)
TagsAPEX2 and V5ExpressionMammalianPromoterCMV-FAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC5Kan-P2A
Plasmid#51814PurposeP2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single messageDepositorInsertP2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)
UseShuttle vector for insect expression vectorsMutationcodons altered for Drosophila and to eliminate re…Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD3 (OTU, aa 52-209)
Plasmid#61412PurposeExpresses human OTUD3 (OTU domain) in E. coli.DepositorInsertOTUD3 (OTUD3 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-51 and aa 210-398.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-BLM
Plasmid#80070PurposeExpresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)DepositorInsertBLM (BLM Human)
TagsGFPExpressionMammalianMutationIf correct ID, the cDNA is from HeLa and has char…Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW-OVOL2-HA
Plasmid#184737PurposeDoxycycline-inducible lentiviral expression of OVOL2DepositorInsertovo like zinc finger 2 (OVOL2 Human)
UseLentiviralTagsHAExpressionMammalianPromoterTet ONAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -