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Showing: 3351 - 3400 of 17836 results

  1. pEasyG3_mic

    Plasmid
    #184920
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184920-plasmid-map-sequence-id-368413

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  2. pGS1034

    Plasmid
    #63796
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1
    Insert
    CBP-3xFLAG-TEV-Protein A followed by KlLEU2
    Use
    E. coli cloning vector
    Tags
    CBP-3xFLAG-TEV-Protein A
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63796-plasmid-map-sequence-id-110311

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  3. pGS1022

    Plasmid
    #59576
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    59576-plasmid-map-sequence-id-92983

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  4. pEasyG2_mic

    Plasmid
    #184916
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184916-plasmid-map-sequence-id-371408

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  9. pUDP010

    Plasmid
    #101166
    Purpose
    E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101166-plasmid-map-sequence-id-199274

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  10. pGS1212

    Plasmid
    #63785
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eGFP-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63785-plasmid-map-sequence-id-106471

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  17. PCUP1-GFP-Pho8-406

    Plasmid
    #207006
    Purpose
    Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.
    Depositor
    Zhiping Xie
    Article
    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.
    Insert
    PHO8
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207006-plasmid-map-sequence-id-422251

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  19. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218291-plasmid-map-sequence-id-433736

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  20. PCUP1-GFP-Osh1-406

    Plasmid
    #207007
    Purpose
    Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.
    Depositor
    Zhiping Xie
    Article
    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.
    Insert
    OSH1
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207007-plasmid-map-sequence-id-422254

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  26. pJR017

    Plasmid
    #198835
    Purpose
    In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::TeTX-SL2-mKate::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198835-plasmid-map-sequence-id-402750

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Showing: 3351 - 3400 of 17836 results