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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,133 results for: puc

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Showing: 3351 - 3400 of 5133 results

  1. pBCL6-donor

    Plasmid
    #112389
    Purpose
    CRISPR donor plasmid to tag human transcription factor BCL6 with GFP
    Depositor
    Kevin White
    Insert
    BCL6 homology arms flanking EGFP-IRES-Neo cassette (BCL6 Human)
    Use
    CRISPR
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112389-plasmid-map-sequence-id-229853

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  2. pE2F5-donor

    Plasmid
    #112351
    Purpose
    CRISPR donor plasmid to tag human transcription factor E2F5 with GFP
    Depositor
    Kevin White
    Insert
    E2F5 homology arms flanking EGFP-IRES-Neo cassette (E2F5 Human)
    Use
    CRISPR
    Mutation
    hg38:8:85213859:T>C, hg38:8:85213860:T>G, h…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112351-plasmid-map-sequence-id-229665

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  3. pZNF445-donor

    Plasmid
    #112332
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF445 with GFP
    Depositor
    Kevin White
    Insert
    ZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
    Use
    CRISPR
    Mutation
    rs78585428, hg38:3:44446218:C>T, hg38:3:444466…
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112332-plasmid-map-sequence-id-228561

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  4. pELK3-donor

    Plasmid
    #112322
    Purpose
    CRISPR donor plasmid to tag human transcription factor ELK3 with GFP
    Depositor
    Kevin White
    Insert
    ELK3 homology arms flanking EGFP-IRES-Neo cassette (ELK3 Human)
    Use
    CRISPR
    Mutation
    hg38:12:96266960:A>T, rs760335647, rs6538704
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112322-plasmid-map-sequence-id-228533

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  36. PB-CT3G-CERP2-MG

    Plasmid
    #175503
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G-mCMV
    Available Since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175503-plasmid-map-sequence-id-345096

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  37. pDAC565

    Plasmid
    #195242
    Purpose
    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    Expression
    Mammalian
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195242-plasmid-map-sequence-id-382950

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  42. pN7HELIX

    Plasmid
    #190662
    Purpose
    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    Expression
    Mammalian
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    CMV
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190662-plasmid-map-sequence-id-385664

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  44. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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Showing: 3351 - 3400 of 5133 results