We narrowed to 13,812 results for: cas9 genes

1. pDONR221 Cas9-RFC4

   Plasmid

   #183199

   Purpose

   Entry clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   Cas9-RFC4
   

   Use

   CRISPR; Gateway entry clone

   Tags

   Expression

   Mutation

   n/a

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330_Tuba1b sgRNA / hSpCas9

   Plasmid

   #172835

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_rSyn1 sgRNA / hSpCas9

   Plasmid

   #172840

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

   Plasmid

   #107731

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

   Plasmid

   #107728

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. BII-ChBtW-eSpCas9

   Plasmid

   #133370

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPD2 dCas9 (GB2664)

   Plasmid

   #160596

   Purpose

   CDS of dCas9 with B3 nomenclature

   Depositor

   Diego Orzaez

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-Intron4-PAFAH1B1

   Plasmid

   #92434

   Purpose

   expresses Cas9 and gRNA targeting PAFAH1B1

   Depositor

   Matthias Leisegang, Ralf Brandes

   Article

   Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

   Insert

   PAFAH1B1 (PAFAH1B1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_UdgX-EE-UdgX-NG-nCas9

   Plasmid

   #163570

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-dCas9-SET7-Hygro

    Plasmid

    #104407

    Purpose

    transient expression of dCas9-SET7 fusion protein

    Depositor

    Stephan Beck

    Insert

    SET7 (SETD7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-H11r1-2-DD-Cas9

    Plasmid

    #85978

    Purpose

    Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    DD-SpCas9
    

    Use

    CRISPR

    Tags

    FKBP12 L106P DD

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBLO 9.6_bCas9-CP1010

    Plasmid

    #121498

    Purpose

    TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p15A

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBLO 9.15_bCas9-CP1249

    Plasmid

    #121500

    Purpose

    TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p15A

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBLO 9.16_bCas9-CP1282

    Plasmid

    #121501

    Purpose

    TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p15A

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBSKΔB-CBh-Cas9n-pA

    Plasmid

    #82617

    Purpose

    Expresses Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    SpCas9n
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-FLAG-eSpCas9

    Plasmid

    #126769

    Purpose

    Expression of increased fidelity eSpCas9 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB_p300_core-dCas9-EGFP_hygro

    Plasmid

    #226431

    Purpose

    PiggyBac transposon vector containing a dox inducible p300 core-dCas9 fusion protein and EGFP reporter. Hygromycin selection marker

    Depositor

    Alon Goren

    Inserts

    p300 core-dCas9-EGFP fusion protein
    rtTA3-IRES-Hygro
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    HA tag, P2A-EGFP, and p300 core

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible minimal CMV and UbC Promoter

    Available since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKDcas9 delta cerulean

    Plasmid

    #230939

    Purpose

    non-targeting Reference Control; expresses mCerulean

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKDcas9 delta mcherry

    Plasmid

    #230940

    Purpose

    non-targeting Reference Control; expresses mCherry

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKDcas9 fis cerulean

    Plasmid

    #230942

    Purpose

    aTc inducible knockdown of Fis

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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