163,347 results

1. HaloTag-bActinCDS-bActinUTR-MS2V5

   Plasmid

   #102718

   Purpose

   Halo-Actin reporter for detection of both mRNA and protein

   Depositor

   Robert Singer

   Article

   Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   HaloTag-bActinCDS-bActin3'UTR-MS2V5
   

   Use

   Lentiviral

   Tags

   HaloTag and MS2V5

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3/Flag-METTL14

   Plasmid

   #53740

   Purpose

   For expression of METTL14 (methyltransferase-like 14) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Methyltransferase-like 14 (METTL14 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-N1-PARP1

   Plasmid

   #211578

   Purpose

   CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAS9-mCherry-Frame +2

   Plasmid

   #66941

   Purpose

   CRISPaint frame selector +2

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA frame +2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV5)

   Viral Prep

   #105540-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   Syn

   Tags

   EGFP

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

6. pRK5-HA-Parkin

   Plasmid

   #17613

   Depositor

   Ted Dawson

   Article

   Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-cmyc

   Plasmid

   #16011

   Purpose

   mammalian expression of human cmyc

   Depositor

   Wafik El-Deiry

   Article

   Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

   Insert

   cmyc (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCI-His-hUbi

   Plasmid

   #31815

   Depositor

   Astar Winoto

   Article

   Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

   Insert

   Ubiquitin (UBB Human)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-ClpX

   Plasmid

   #234733

   Purpose

   Human-codon optimized E. coli ClpX in mammalian cells

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   ClpX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-mDlx-GCaMP6f-Fishell-2 (AAV1)

    Viral Prep

    #83899-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-mDlx-GCaMP6f-Fishell-2 (#83899). In addition to the viral particles, you will also receive purified pAAV-mDlx-GCaMP6f-Fishell-2 plasmid DNA. mDlx-driven expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    mDlx

    Tags

    None

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

11. pLV GG hUbC-dsRED

    Plasmid

    #84034

    Purpose

    Contains Golden Gate cassette to express up to four gRNAs and dsRED

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pink Flamindo

    Plasmid

    #102356

    Purpose

    biosensor for cAMP in mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

    Insert

    Pink Flamindo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209730

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 4xNFkB Luc

    Plasmid

    #111216

    Purpose

    reporter gene

    Depositor

    Johannes A. Schmid

    Insert

    firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti6.2-ccdB-Nanoluc

    Plasmid

    #87075

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SpyCatcher003 S49C

    Plasmid

    #133448

    Purpose

    Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Contains S49C mutation

    Promoter

    T7

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RBD-Fc

    Plasmid

    #164221

    Purpose

    Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike RBD (S SARS-CoV)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNP1950

    Plasmid

    #222729

    Purpose

    J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    David Root, John Doench, William Hahn

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-Cre-P2A-dTomato (AAV PHP.eB)

    Viral Prep

    #107738-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Rylan Larsen

    Promoter

    Syn

    Tags

    dTomato (physically separate, not a fusion protein)

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

21. PB-TRE-EGFP-EF1a-rtTA

    Plasmid

    #104454

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

    Depositor

    Volker Busskamp

    Article

    Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

    Inserts

    enhanced green fluorescent protein
    reverse tetracycline-controlled transactivator 3
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSP6-RNAP

    Plasmid

    #48143

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase SP6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    V314A (numbering based on NCBI Sequence ID: NP_85…

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mCherry-Golgi-7

    Plasmid

    #55052

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-EGFP-Cdc42(Q61L)

    Plasmid

    #12600

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (Q61L) (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. C321.ΔA.exp

    Bacterial Strain

    #49018

    Purpose

    This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Bleocin (zeocin)

    Species

    Escherichia coli

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

26. YE1-BE4max

    Plasmid

    #138155

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV9)

    Viral Prep

    #26969-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

    Promoter

    CaMKII

    Tags

    EYFP

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

28. EB3-tdTomato

    Plasmid

    #50708

    Purpose

    expresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

    Depositor

    Erik Dent

    Article

    Merriam et al J Neurosci. 2013 Oct 16;33(42):16471-82.

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSyn-mCherry (AAV8)

    Viral Prep

    #114472-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Syn

    Tags

    mCherry

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

30. FU-H2B-GFP-IRES-Puro

    Plasmid

    #69550

    Purpose

    Lentiviral, selectable fluorescent GFP tagging of nuclei.

    Depositor

    Charles Gersbach

    Insert

    Histone 2B - green fluorescent protein fusion
    

    Use

    Lentiviral

    Tags

    GFP fusion

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge