We narrowed to 10,328 results for: pcas

1. pCAG-T3-hCasD10A-pA

   Plasmid

   #51638

   Purpose

   Expresses Cas9-nickase in mammalian cells and zygotes.

   Depositor

   Wataru Fujii

   Article

   Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

   Insert

   Codon-optimized Cas9 D10A
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCAG-mTHC

   Plasmid

   #112620

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-mTFP1 (H2BC3 Anthozoa, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Tags

   mTeal (mTFP1)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-hPPP3R1

   Plasmid

   #179136

   Purpose

   Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

   Plasmid

   #188541

   Purpose

   Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-GCaMP2

   Plasmid

   #18926

   Depositor

   Karel Svoboda

   Article

   Mao et al PLoS ONE. 2008 . 3(3):e1796.

   Insert

   GCaMP2
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pCAGGS-FuG-B

   Plasmid

   #67523

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al Hum Gene Ther. 2011 Feb;22(2):197-206. d

   Insert

   Fusion Glycoprotein type B
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAT.015

   Plasmid

   #119558

   Purpose

   Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. UVR8-GFPCaaX

   Plasmid

   #49805

   Purpose

   expresses UVR8 fused to GFP with a prenylation motif for membrane targeting

   Depositor

   Matthew Kennedy

   Article

   Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

   Insert

   UVR8-GFPCaax (UVR8 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAT.000

   Plasmid

   #119559

   Purpose

   Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCALNL-mutKir2.1

    Plasmid

    #125579

    Purpose

    Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A non-conducting mutant (GYG -> AAA).

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-6017nes

    Plasmid

    #108655

    Purpose

    A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-PKA-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-6015nes

    Plasmid

    #108657

    Purpose

    A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-S6K-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-T3-hCASeGFP-pA

    Plasmid

    #59766

    Purpose

    Expresses CAS9-eGFP fusion protein in mammalian cells.

    Depositor

    Wataru Fujii

    Article

    Fujii et al Nucleic Acids Res. 2013 Aug 30.

    Insert

    CAS9
    

    Use

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAP03-act1p

    Plasmid

    #120233

    Purpose

    Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing act1p promoter and apramycin resistance gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    aac(3)IV (apramycin resistance gene)

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas9-J23109

    Plasmid

    #113150

    Purpose

    Plasmid constitutively expressing Cas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Ssh1

    Plasmid

    #163602

    Purpose

    Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    ssh1 (Ssh1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG.Myc-SARS2_3CL

    Plasmid

    #200120

    Purpose

    Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG/ATP10B-HA

    Plasmid

    #209216

    Purpose

    Mammalian expression of ATP10B

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCALNL-Kir2.1

    Plasmid

    #125578

    Purpose

    Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1a-nSpCas9-NG

    Plasmid

    #171692

    Purpose

    Expresses nSpCas9-NG in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    nSpCas9-NG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

    Promoter

    EF1a

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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