We narrowed to 92,818 results for: plasmid
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Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only
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LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 hygro
Plasmid#104995PurposeThis lentiviral construct delivers hSpCas9 and hygromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 hygro
Plasmid#104995PurposeThis lentiviral construct delivers hSpCas9 and hygromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII 0.4.Cre.SV40 (AAV5)
Viral Prep#105558-AAV5PurposeReady-to-use AAV5 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIIAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (AAV9)
Viral Prep#100848-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro LEF1
Plasmid#27023DepositorAvailable SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro LEF1
Plasmid#27023DepositorAvailable SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRMC2
Plasmid#68940Purposetetracycline-inducible expression vector for Staphylococcus aureusDepositorTypeEmpty backboneUseTet inducible e. coli/staphylococcus shuttle vect…ExpressionBacterialAvailable SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAKgfp1
Plasmid#14076DepositorInsertGreen fluorescent protein
ExpressionBacterialAvailable SinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flpe
Plasmid#13787PurposeMammalian expression of enhanced form of Flp recombinase with C-terminal Myc tagDepositorInsertFlpe
TagsMycExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEFCas9
Plasmid#87115PurposeSpCas9 expression AAV backbone plasmid under control of nEF promoterDepositorInsertnEF-Cas9
UseAAV and CRISPRTagsHA-NLSExpressionMammalianAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hSOX2
Plasmid#17218PurposeRetroviral expression of human SOX2DepositorAvailable SinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNGN2
Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsTagsT2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and TRE3GAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cre Stoplight 2.4
Plasmid#37402PurposeCre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with CreDepositorInsertCre Stoplight 2.4
TagsZsGreen1 and mCherryExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
Cre Stoplight 2.4
Plasmid#37402PurposeCre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with CreDepositorInsertCre Stoplight 2.4
TagsZsGreen1 and mCherryExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTP1174
Plasmid#104162PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TLCV2
Plasmid#87360PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.DepositorHas ServiceCloning Grade DNAInsertCas9-2A-eGFP
UseCRISPR and Lentiviral; Doxycycline inducible; egf…TagsCas9-T2A-eGFPExpressionMammalianPromoterTight TRE promoterAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6f (AAV1)
Viral Prep#128315-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-GCaMP6f (#128315). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-GCaMP6f plasmid DNA. EF1a-driven, Flp-dependent expression of calcium sensor GCaMP6f. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-HA
Plasmid#141320PurposePlasmid to carry out IVT of RfxCas13d (human codon-optimized)DepositorInsertRfx-Cas13d
UseCRISPRTagsHAAvailable SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only