Skip to main content
Addgene
Showing: 3081 - 3120 of 8751 results

  1. pDY0973-RADARS iCaspase

    Plasmid
    #182539
    Purpose
    Express RADARS iCaspase that conditionally turn on iCaspase in the presence of target RNA
    Depositor
    Jonathan Gootenberg, Omar Abudayyeh
    Insert
    iCaspase9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182539-plasmid-map-sequence-id-356646

    Enlarge

  2. pJY4092

    Plasmid
    #113334
    Purpose
    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter
    Depositor
    Suzanne Sandmeyer
    Insert
    codon-optimized Hermes transposase
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113334-plasmid-map-sequence-id-218285

    Enlarge

  3. piggyBAC-U6-GFP-WPRE

    Plasmid
    #200030
    Purpose
    piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
    Depositor
    Jay Shendure
    Type
    Empty backbone
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    200030-plasmid-map-sequence-id-393204

    Enlarge

  4. BII-Sh-BnTW

    Plasmid
    #133365
    Purpose
    Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    Flag-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133365-plasmid-map-sequence-id-268571

    Enlarge

  5. pCMX-Gal4-TEAD1-D278K

    Plasmid
    #38106
    Depositor
    Kunliang Guan
    Insert
    TEAD1 (TEAD1 Human)
    Use
    Tags
    Gal4-DBD
    Expression
    Mammalian
    Mutation
    D278K
    Promoter
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  6. pLV Rnf121-V5

    Plasmid
    #175130
    Purpose
    Lentiviral expression of mouse Rnf121-V5
    Depositor
    Larry Gerace
    Insert
    Rnf121 (Rnf121 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175130-plasmid-map-sequence-id-352014

    Enlarge

  7. pLV V5-Mospd1

    Plasmid
    #175132
    Purpose
    Lentiviral expression of V5-tagged mouse Mospd1
    Depositor
    Larry Gerace
    Insert
    Mospd1 (Mospd1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175132-plasmid-map-sequence-id-352019

    Enlarge

  8. pSBbi-pur H-2Kb

    Plasmid
    #111623
    Purpose
    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele
    Depositor
    Jon Yewdell
    Insert
    H2Kb
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111623-plasmid-map-sequence-id-213220

    Enlarge

  9. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Daniel Geschwind
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201999-plasmid-map-sequence-id-398468

    Enlarge

  11. pDY0551-Luciferase RADARS

    Plasmid
    #182538
    Purpose
    Mammalian cell expression plasmid of Gaussia luciferase RADARS in a dual luciferase expression back bone with Cypridina luciferase as the control gene.
    Depositor
    Jonathan Gootenberg, Omar Abudayyeh
    Insert
    RADARS Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182538-plasmid-map-sequence-id-356645

    Enlarge

  12. pLV V5-Nesprin-3

    Plasmid
    #175136
    Purpose
    Lentiviral expression of V5-tagged mouse Syne3
    Depositor
    Larry Gerace
    Insert
    Syne3 (Syne3 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175136-plasmid-map-sequence-id-352023

    Enlarge

  13. pLV Nup62-V5

    Plasmid
    #175116
    Purpose
    Lentiviral expression of mouse Nup62-V5
    Depositor
    Larry Gerace
    Insert
    Nup62 (Nup62 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175116-plasmid-map-sequence-id-351797

    Enlarge

  14. pLV Tor1a-V5

    Plasmid
    #175135
    Purpose
    Lentiviral expression of Tor1a-Myc
    Depositor
    Larry Gerace
    Insert
    Tor1a (Tor1a Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175135-plasmid-map-sequence-id-352022

    Enlarge

  15. pMC6-TEFkFLP(LYS2)

    Plasmid
    #49458
    Purpose
    overespression of Flp step-arrest mutant in yeast, LYS2 selection
    Depositor
    Marc Gartenberg
    Insert
    Flp(H305L) (flp Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L, ‘step-arrest’ mutant,
    Promoter
    TEF1
    Available since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    49458-plasmid-map-sequence-id-73670

    Enlarge

  16. pLV Nup88-V5

    Plasmid
    #175117
    Purpose
    Lentiviral expression of mouse Nup88-V5
    Depositor
    Larry Gerace
    Insert
    Nup88 (Nup88 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175117-plasmid-map-sequence-id-351804

    Enlarge

  17. pLV Tyw1-V5

    Plasmid
    #175131
    Purpose
    Lentiviral expression of mouse Tyw1-V5
    Depositor
    Larry Gerace
    Insert
    Tyw1 (Tyw1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175131-plasmid-map-sequence-id-352017

    Enlarge

  18. pLV Rbmxl1-V5

    Plasmid
    #175124
    Purpose
    Lentiviral expression of mouse Rbmxl1-V5
    Depositor
    Larry Gerace
    Insert
    Rbmxl1 (Rbmxl1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175124-plasmid-map-sequence-id-351936

    Enlarge

  19. lenti-RfxCas13d-Blast-BFP

    Plasmid
    #196726
    Purpose
    Sortable and selectable RfxCas13d.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196726-plasmid-map-sequence-id-389737

    Enlarge

  20. lenti.Cas9.BFP.Blast

    Plasmid
    #196714
    Purpose
    WT SpCas9 with BSD-TagBFP for selection and sorting
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196714-plasmid-map-sequence-id-388043

    Enlarge

  21. pLV V5-Sun2

    Plasmid
    #175114
    Purpose
    Lentiviral expression of V5-tagged mouse Sun2
    Depositor
    Larry Gerace
    Insert
    Sun2 (Sun2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175114-plasmid-map-sequence-id-351772

    Enlarge

  22. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid
    #171939
    Purpose
    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein
    Depositor
    David Berson
    Insert
    APEX2-eGFPf
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171939-plasmid-map-sequence-id-338504

    Enlarge

  23. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

    Enlarge

  24. pSBbi-Pur-AktAR2

    Plasmid
    #125195
    Purpose
    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125195-plasmid-map-sequence-id-243600

    Enlarge

  25. pLV Tmx1-V5

    Plasmid
    #175138
    Purpose
    Lentiviral expression of V5-tagged mouse Tmx1
    Depositor
    Larry Gerace
    Insert
    Tmx1 (Tmx1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175138-plasmid-map-sequence-id-352052

    Enlarge

  26. pLV Tmem9b-V5

    Plasmid
    #175152
    Purpose
    Lentiviral expression of mouse Tmem9b-V5
    Depositor
    Larry Gerace
    Insert
    Tmem9b (Tmem9b Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    175152-plasmid-map-sequence-id-352193

    Enlarge

  27. pLV Ndc1-V5

    Plasmid
    #175115
    Purpose
    Lentiviral expression of mouse Ndc1-V5
    Depositor
    Larry Gerace
    Insert
    Ndc1 (Ndc1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175115-plasmid-map-sequence-id-351773

    Enlarge

  28. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

    Enlarge

  29. pLV V5-Sun1

    Plasmid
    #175113
    Purpose
    Lentiviral expression of V5-tagged mouse Sun1
    Depositor
    Larry Gerace
    Insert
    Sun1 (Sun1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175113-plasmid-map-sequence-id-351771

    Enlarge

  30. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

    Enlarge

  32. lenti-UCOE-dCas9-BFP-VP64

    Plasmid
    #196717
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196717-plasmid-map-sequence-id-388038

    Enlarge

  33. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

    Enlarge

  34. lenti-dCas9-VP64-BFP

    Plasmid
    #196711
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-VP64-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196711-plasmid-map-sequence-id-392561

    Enlarge

  35. Gal4-TEAD4

    Plasmid
    #24640
    Depositor
    Kunliang Guan
    Insert
    TEAD-4 (TEAD4 Human)
    Use
    Tags
    Gal4
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2010
    Availability
    Academic Institutions and Nonprofits only

  36. pLV-Puro_hCOL4A4

    Plasmid
    #198099
    Purpose
    lentiviral expression of hCOL4A4 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A4 (COL4A4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    198099-plasmid-map-sequence-id-389063

    Enlarge

  37. pLV Tor1a-Myc

    Plasmid
    #175134
    Purpose
    Lentiviral expression of Myc-tagged mouse Tor1a
    Depositor
    Larry Gerace
    Insert
    Tor1a (Tor1a Mouse)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175134-plasmid-map-sequence-id-352021

    Enlarge

  38. pLV V5-Senp2

    Plasmid
    #175118
    Purpose
    Lentiviral expression of V5-tagged mouse Senp2
    Depositor
    Larry Gerace
    Insert
    Senp2 (Senp2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175118-plasmid-map-sequence-id-351807

    Enlarge

  39. pCAS9id_TRP

    Plasmid
    #141254
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141254-plasmid-map-sequence-id-308894

    Enlarge

  40. pSBBi-RP CCS1

    Plasmid
    #139162
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    caffeine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139162-plasmid-map-sequence-id-309352

    Enlarge

Showing: 3081 - 3120 of 8751 results