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Showing: 3901 - 3950 of 11732 results

  1. pGBW-m4046381

    Plasmid
    #149010
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149010-plasmid-map-sequence-id-297723

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  2. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7

    Plasmid
    #214140
    Purpose
    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    Tags
    2xSTII
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214140-plasmid-map-sequence-id-431241

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  3. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121537-plasmid-map-sequence-id-235299

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  7. pGBW-m4046277

    Plasmid
    #145428
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145428-plasmid-map-sequence-id-298356

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  8. pAL612

    Plasmid
    #119860
    Purpose
    precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
    Tags
    none
    Expression
    Bacterial
    Mutation
    Cysterine 290 to Serine and Glycine 244 to Cystei…
    Promoter
    lac
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119860-plasmid-map-sequence-id-232053

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  9. pAM5085

    Plasmid
    #85122
    Purpose
    PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
    Tags
    ECFP
    Expression
    Bacterial
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85122-plasmid-map-sequence-id-180108

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  13. pJL NarL YdfI Flag

    Plasmid
    #225650
    Purpose
    Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NarL-YdfI (narL Synthetic)
    Use
    Cell free expression
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    NarL with native DNA-binding domain replaced with…
    Promoter
    T7
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    225650-plasmid-map-sequence-id-454890

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  15. pGBW-m4046216

    Plasmid
    #145490
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145490-plasmid-map-sequence-id-298323

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  16. pGBW-m4046268

    Plasmid
    #145423
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145423-plasmid-map-sequence-id-298350

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  18. CFF-EGFP

    Plasmid
    #86061
    Purpose
    A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains luminal and transmembrane domains of CD8…
    Promoter
    CMV
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86061-plasmid-map-sequence-id-177941

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  19. NST1_pOpOn2.1

    Plasmid
    #228065
    Purpose
    Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.
    Depositor
    Charles Anderson
    Article
    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10
    Insert
    NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
    Use
    Dexamethasone inducible vector
    Expression
    Plant
    Promoter
    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1
    Available Since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228065-plasmid-map-sequence-id-456794

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  20. pSN-A112C-GpA

    Plasmid
    #191238
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191238-plasmid-map-sequence-id-380469

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  21. CD8a-f-CTS-EGFP

    Plasmid
    #86063
    Purpose
    A mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains CD8a luminal and transmembrane domains a…
    Promoter
    CMV
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86063-plasmid-map-sequence-id-177944

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  22. CD8a-hnRNP-M-EGFP

    Plasmid
    #86054
    Purpose
    A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    CD8a (CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    CD8a luminal and transmembrane domians along with…
    Promoter
    CMV
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86054-plasmid-map-sequence-id-177920

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  23. pCDNA3.1-nAChr-beta3-YFP

    Plasmid
    #50484
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    YFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. pSN-A112C-GpA-G83I

    Plasmid
    #191239
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and Glycin…
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191239-plasmid-map-sequence-id-380471

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  25. CD8a-cNLS-EGFP

    Plasmid
    #86052
    Purpose
    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    CD8a (CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    CD8a luminal and transmembrane along with two SV4…
    Promoter
    CMV
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86052-plasmid-map-sequence-id-177916

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  26. pGBW-m4136462

    Plasmid
    #149533
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149533-plasmid-map-sequence-id-307560

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  27. pGBW-m4046763

    Plasmid
    #145606
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145606-plasmid-map-sequence-id-298330

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  35. pOPARA1

    Plasmid
    #200416
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200416-plasmid-map-sequence-id-394143

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  46. GGE020

    Plasmid
    #120777
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position1-3. When using the GG platform for expressing only one gene (T1-3_Lip2)
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    T1-3_Lip2
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120777-plasmid-map-sequence-id-263162

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  49. GGE176

    Plasmid
    #120733
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Auxotrophy marker for selection after transformation in Y. lipolytica (Marker_Lys5)
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    Marker_Lys5
    Use
    Cloning/assembly plasmid
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    120733-plasmid-map-sequence-id-259143

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Showing: 3901 - 3950 of 11732 results