We narrowed to 72,121 results for: LAS

1. pLAS3W-S100P

   Plasmid

   #125009

   Purpose

   express in mammalian cell

   Depositor

   Ruey-Hwa Chen

   Article

   Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

   Insert

   S100P (S100P Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRi(v1)-Blast

   Plasmid

   #170067

   Purpose

   Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   KRAB-dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2A and 2xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. TPI in pCW57_tTA_Blast

   Plasmid

   #154209

   Purpose

   Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   TPI1 (TPI1 Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   TRE repeats (dox off)

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiMutB3GNT5-blast

   Plasmid

   #208380

   Purpose

   lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   nt 922 c->g (PAM site inactivation)

   Promoter

   EF-1 alpha core promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiMutB3GALT5-blast

   Plasmid

   #208381

   Purpose

   lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   nucleotide change in PAM targeting sequence nt 51…

   Promoter

   EF-1 alpha core promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiMutB3GALT5catdead-blast

   Plasmid

   #220869

   Purpose

   lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   encodes D156A and D243A mutations; also, nucleoti…

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINS-Blast

   Plasmid

   #31388

   Depositor

   Yegor Vassetzky

   Article

   Dmitriev et al BMC Res Notes. 2008 . 1():135.

   Insert

   bsd
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-subtilase A

   Plasmid

   #35948

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   Subtilase A (subA E. Coli)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pLux76-LasI

   Plasmid

   #73444

   Purpose

   HSL relay device: produces 3O-C12-HSL in response to 3O-C6-HSL

   Depositor

   Jim Haseloff

   Article

   Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

   Insert

   LasI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pLux76

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLas81-LuxI

    Plasmid

    #73445

    Purpose

    HSL relay device: produces 3O-C6-HSL in response to 3O-C12-HSL

    Depositor

    Jim Haseloff

    Article

    Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

    Insert

    LuxI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLas81

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Accessory plasmid 1

    Plasmid

    #128569

    Purpose

    for mixing with SPECS library to produce lentivirus

    Depositor

    Timothy Lu

    Article

    Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

    Insert

    ECFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Rlucrarr3-Flash6

    Plasmid

    #74134

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. Accessory plasmid 2

    Plasmid

    #128571

    Purpose

    for cloning identified individual SPECS

    Depositor

    Timothy Lu

    Article

    Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagRFP

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV+ plasmid

    Plasmid

    #169740

    Purpose

    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mc2 blast

    Plasmid

    #206217

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWZL Blast ST

    Plasmid

    #13805

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    SV40 small T antigen (SV40gp7 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

17. Rlucrarr3-Flash1

    Plasmid

    #74129

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pLV-Blast_hCOL4A5-HA

    Plasmid

    #198103

    Purpose

    lentiviral expression of hCOL4A5 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Rlucrarr3-Flash4

    Plasmid

    #74132

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

20. Rlucrarr3-Flash3

    Plasmid

    #74131

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only