We narrowed to 72,985 results for: LAS

1. Equalizer-M plasmid

   Plasmid

   #169731

   Purpose

   Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Equalizer-H plasmid

   Plasmid

   #169367

   Purpose

   Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE30-butelase1

   Plasmid

   #115430

   Purpose

   Expresses the macrocyclizing asparaginyl endopeptidase butelase 1 in E. coli

   Depositor

   Joshua Mylne

   Article

   James et al Plant J. 2019 Feb 21. doi: 10.1111/tpj.1

   Insert

   butelase 1
   

   Use

   Tags

   6-His

   Expression

   Mutation

   Promoter

   T5

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEED-OLLAS:HA

   Plasmid

   #219647

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   OLLAS:HA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Bsrs078-LasR

   Plasmid

   #85147

   Purpose

   Constitutively expresses LasR

   Depositor

   Jeff Hasty

   Article

   Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

   Insert

   LasR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Blast_hCOL4A5

   Plasmid

   #198100

   Purpose

   lentiviral expression of hCOL4A5 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A5 (COL4A5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3XFLAG_ALDOA in pCW57_tTA_Blast

   Plasmid

   #154210

   Purpose

   Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of ALDOA

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE repeats (dox off)

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWZLblasti-CTR1

   Plasmid

   #53157

   Purpose

   Expresses human CTR1 from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   CTR1 (SLC31A1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

9. Bsrs074-Plas

   Plasmid

   #85155

   Purpose

   Inducibile Las Promoter driving sfGFP

   Depositor

   Jeff Hasty

   Article

   Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

   Insert

   Plas-sfGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZLblasti-HA-ERK5

    Plasmid

    #53175

    Purpose

    Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK5 (MAPK7 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

11. TPI in pCW57_tTA_Blast

    Plasmid

    #154209

    Purpose

    Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    TPI1 (TPI1 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    TRE repeats (dox off)

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLAS3W-S100P

    Plasmid

    #125009

    Purpose

    express in mammalian cell

    Depositor

    Ruey-Hwa Chen

    Article

    Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

    Insert

    S100P (S100P Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLux76-LasI

    Plasmid

    #73444

    Purpose

    HSL relay device: produces 3O-C12-HSL in response to 3O-C6-HSL

    Depositor

    Jim Haseloff

    Article

    Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

    Insert

    LasI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLux76

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pINS-Blast

    Plasmid

    #31388

    Depositor

    Yegor Vassetzky

    Article

    Dmitriev et al BMC Res Notes. 2008 . 1():135.

    Insert

    bsd
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-subtilase A

    Plasmid

    #35948

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    Subtilase A (subA E. Coli)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

16. lentiMutB3GNT5-blast

    Plasmid

    #208380

    Purpose

    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nt 922 c->g (PAM site inactivation)

    Promoter

    EF-1 alpha core promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiMutB3GALT5-blast

    Plasmid

    #208381

    Purpose

    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nucleotide change in PAM targeting sequence nt 51…

    Promoter

    EF-1 alpha core promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiMutB3GALT5catdead-blast

    Plasmid

    #220869

    Purpose

    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    encodes D156A and D243A mutations; also, nucleoti…

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLas81-LuxI

    Plasmid

    #73445

    Purpose

    HSL relay device: produces 3O-C6-HSL in response to 3O-C12-HSL

    Depositor

    Jim Haseloff

    Article

    Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

    Insert

    LuxI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLas81

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Accessory plasmid 2

    Plasmid

    #128571

    Purpose

    for cloning identified individual SPECS

    Depositor

    Timothy Lu

    Article

    Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagRFP

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge