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pVC-Ds-DrU6a:scrambled3-sgRNA-Ds

Plasmid

#119072

Purpose

Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled3-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled4-sgRNA-Ds

Plasmid

#119073

Purpose

Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled4-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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ins:BB1.0L; cryaa:RFP

Plasmid

#110283

Purpose

A zebrafish insulin promoter driving the Brainbow (BB1.0L) cassette. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Insert

Brainbow1.0L

Use

Zebrafish

Promoter

insulin

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

Plasmid

#108871

Purpose

Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Inserts

5xU6:sgRNA
Cas9-t2A-GFP

Use

CRISPR

Promoter

U6 and hsp70

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-mRuby-Rab8a

Plasmid

#194337

Purpose

Expresses mRuby Rab8a in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Rab8 (RAB8A Canis lupus)

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Cs2+Etv4(pea3)

Plasmid

#49093

Purpose

expression of Etv4

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv4 (etv4 Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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Cs2+Etv5

Plasmid

#49095

Purpose

expression of Etv5

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv5 (etv5a Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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p5E-mpeg1.1

Plasmid

#75023

Purpose

Can be used to drive expression in zebrafish microglia.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

mpeg1.1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-nCas9n-nanos 3’UTR

Plasmid

#62542

Purpose

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

nanos 3' UTR (nanos3 Zebrafish)

Use

CRISPR

Mutation

3' UTR only

Promoter

SP6

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pMT-HuC:Dendra2

Plasmid

#80904

Purpose

Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2

Depositor

Periklis Pantazis

Article

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

Dendra2 (elavl3 Synthetic)

Use

Zebrafish expression

Promoter

HuC

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-Cas9

Plasmid

#47322

Purpose

For in vivo expression of Cas9 or generation of Cas9 mRNA

Depositor

Alex Schier

Article

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR; Xenopus and zebrafish expression

Expression

Mammalian

Promoter

SP6

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pT3TS nErCas12an

Plasmid

#132642

Purpose

Used for in vitro transcription of dual NLS ErCas12a mRNA

Depositor

Stephen Ekker

Article

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

ErCas12a

Use

CRISPR; In vitro transcription

Tags

SV40 NLS

Promoter

T3

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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ins:MCS2; cryaa:RFP

Plasmid

#110286

Purpose

A zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Type

Empty backbone

Use

Zebrafish

Promoter

insulin

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcna1a-IRES-EGFP

Plasmid

#164965

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcna1a (kcna1a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP

Plasmid

#194696

Purpose

HuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafish

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Use

Zebrafish expression

Tags

mEGFP

Promoter

elavl3(HuC)

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+PIF6CreN

Plasmid

#131780

Purpose

Mammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite system

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PIF6CreN

Use

Cre/Lox; Zebrafish expression

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+PhyBΔCreC

Plasmid

#131781

Purpose

Mammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC

Use

Cre/Lox; Zebrafish expression

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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ins:MCS; cryaa:RFP

Plasmid

#110282

Purpose

A zebrafish insulin promoter containing vector with multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Type

Empty backbone

Use

Zebrafish

Promoter

insulin

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

Plasmid

#67668

Purpose

Zebrafish expression vector for ubiquitous expression of APEX2-GBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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