We narrowed to 19,679 results for: ACE

1. pProm1_BCD1-GFP

   Plasmid

   #80649

   Purpose

   Promoter1 with BCD1-gfp

   Depositor

   Nathan Hillson

   Article

   Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

   Insert

   GFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   NONE

   Promoter

   constitutive promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. DVK_AF

   Plasmid

   #66068

   Purpose

   MoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Type

   Empty backbone

   Use

   Synthetic Biology; Destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pICSL70008

   Plasmid

   #50336

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMRMTK-clo10

   Plasmid

   #203961

   Purpose

   Cloning plasmid containing the erythromycin resistance gene insert

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   Erythromycin Resistance Gene
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   N/A

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGWT35S-Cerulean-SKL

   Plasmid

   #194048

   Purpose

   Transient expression of Cerulean-SKL in plant cell (Peroxisome)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   Cerulean
   

   Use

   Tags

   Ser-Lsy-Leu

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL70004

   Plasmid

   #50334

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   kanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Myr.PKCzeta.FLAG

   Plasmid

   #10802

   Depositor

   Alex Toker

   Article

   Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

   Insert

   PKC zeta (Prkcz Rat)
   

   Use

   Tags

   FLAG and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pMC-XI2

   Plasmid

   #176156

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQLinkHD_EBFP2-LSSmOrange

   Plasmid

   #118856

   Purpose

   fluorescent protein expression with an N-terminal His-tag

   Depositor

   Marc Birtwistle

   Article

   Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

   Insert

   EBFP2-LSSmOrange
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   EBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…

   Promoter

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5/FRT/TO_TOMM20-STf

    Plasmid

    #182002

    Purpose

    CMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    TOMM20-STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221 Spinophilin

    Plasmid

    #87123

    Purpose

    Gateway donor vector for Spinophilin

    Depositor

    Anthony Baucum

    Article

    Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

    Insert

    Spinophilin (PPP1R9B Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pgRNA-non-target

    Plasmid

    #129465

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Non-target/random gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET29b-dspB(E184Q)-6xHis

    Plasmid

    #175802

    Purpose

    Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUPD2+E-Luc

    Plasmid

    #170873

    Purpose

    Phytobrick - E-Luc Luciferase CDS

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    c.375C>T to remove BbsI site

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRYG

    Plasmid

    #113642

    Purpose

    Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    mRFP1-linker-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAurora

    Plasmid

    #190158

    Purpose

    Regulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.

    Depositor

    Andreas Moeglich

    Article

    Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

    Type

    Empty backbone

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCrepusculo

    Plasmid

    #190156

    Purpose

    Regulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.

    Depositor

    Andreas Moeglich

    Article

    Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

    Type

    Empty backbone

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFTK087

    Plasmid

    #171359

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    P-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACS130

    Plasmid

    #204595

    Purpose

    Histone H3.1K9M-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    histone H3.1 (H3C3 Human)
    

    Use

    Lentiviral

    Tags

    human histone H3.1K9M-V5

    Expression

    Mammalian

    Mutation

    H3.1K9M

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 4xHRE_minSV40-sfGFP-CMV_dsRed

    Plasmid

    #117400

    Purpose

    sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMC-X4

    Plasmid

    #176154

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMC-XII1

    Plasmid

    #176159

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMC-XI3

    Plasmid

    #176157

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQcre1

    Plasmid

    #135659

    Purpose

    Clostridial vector, encoding the Cre protein, which mediates recombination between lox sites.

    Depositor

    Andrew Tolonen

    Article

    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ppag promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMZ1001

    Plasmid

    #204398

    Purpose

    A pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

    Inserts

    quorum sensing regulator RpaR (rpaR )
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PftsH and Synthetic promoter PRpa0002

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMC-XII5

    Plasmid

    #176161

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pre_II_CS3_7560_v1

    Plasmid

    #126853

    Purpose

    7560 b scaffold from compatibility group 3, made by method II

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    II_CS3_7560_v1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJ204-loxP-100RPU.2-coEPO-lox2272

    Plasmid

    #154828

    Purpose

    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    coEPO
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized (Cricetulus griseus)

    Promoter

    100RPU.2

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pWXLd-RIEP-OgNLuc

    Plasmid

    #135936

    Purpose

    Expresses the OgNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

    Plasmid

    #190794

    Purpose

    Template vector to amplify pieces for creating multiplex sgRNA-arrays.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA-scaffold only
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23117

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ChlorON-2

    Plasmid

    #193478

    Purpose

    Turn-on fluorescent sensor for chloride

    Depositor

    Sheel Dodani

    Article

    Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

    Insert

    ChlorON-2
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid

    #111926

    Purpose

    Cyan color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_1.2μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pICSL50015

    Plasmid

    #50318

    Purpose

    Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mNEON (Branchistoma lanceolatum)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC19, containing H2-Kd Balb/c WK10

    Plasmid

    #102642

    Purpose

    Encodes sequence of the murine H2-Kd allele

    Depositor

    Sai Reddy

    Article

    Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

    Insert

    H2-Kd Balb/c
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TRF2-HA

    Plasmid

    #200209

    Purpose

    Expression of full length mTRF2

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TRF2-HA (Terf2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-hSYN1-fDIO-rtTA

    Plasmid

    #166598

    Purpose

    Encodes Flp-dependent rtTA

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    rtTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human synapsin 1

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Pr-deGFP

    Plasmid

    #67743

    Purpose

    Pr promoter expressing deGFP

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pWKS1583

    Plasmid

    #70188

    Purpose

    Modular vector for secreted amylase reporter expression

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

    Insert

    amyEopt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pICH87633

    Plasmid

    #50271

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. orthodenticle

    Plasmid

    #37285

    Depositor

    Norbert Perrimon

    Article

    Finkelstein et al Genes Dev. 1990 Sep;4(9):1516-27.

    Insert

    orthodenticle (oc Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only