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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,612 results for: eng

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Showing: 1701 - 1720 of 22612 results

  2. AICSDP-4:TUBA1B-mEGFP

    Plasmid
    #87421
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    87421-plasmid-map-sequence-id-441601

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  6. 803 pGEX2T PTEN

    Plasmid
    #10784
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    GST
    Expression
    Bacterial
    Available since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only

  7. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid
    #140005
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140005-plasmid-map-sequence-id-275488

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  16. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

    Plasmid
    #164510
    Purpose
    Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.hSynap.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244092) instead of this plasmid.
    Depositor
    Loren Looger
    Publication
    Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi
    Has service
    AAV9
    Insert
    (Cyto)iGlucoSnFR-mRuby2
    Use
    AAV
    Mutation
    Synthetic Construct
    Promoter
    Synapsin
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164510-plasmid-map-sequence-id-323856

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  18. pAAVS1-P-CAG-GFPluc2

    Plasmid
    #80493
    Purpose
    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression
    Depositor
    Knut Woltjen
    Publication
    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFPluc2
    Use
    Donor vector (human)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80493-plasmid-map-sequence-id-151598

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Showing: 1701 - 1720 of 22612 results