Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 8,813 results for: Ott

Showing: 4551 - 4600 of 8813 results

  3. pPLN-hFluc-DM-eGFP-KDEL

    Plasmid
    #177728
    Purpose
    DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFlucDM
    Use
    Tags
    KDEL, Prolactin Signal Sequence, and eGFP
    Expression
    Bacterial and Mammalian
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177728-plasmid-map-sequence-id-352602

    Enlarge

  4. IX301: pMVP (L3-L2) myc epitope tag + polyA

    Plasmid
    #121753
    Purpose
    pMVP L3-L2 entry plasmid, contains myc epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    myc epitope tag + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121753-plasmid-map-sequence-id-237192

    Enlarge

  9. LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA

    Plasmid
    #47453
    Purpose
    epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE (N136,Grm2,C63)-cib1
    Use
    AAV; Tale
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47453-plasmid-map-sequence-id-72982

    Enlarge

  11. pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47450
    Purpose
    AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5HD_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47450-plasmid-map-sequence-id-69442

    Enlarge

  12. IX601: pMVP (L3-L2) FLAG epitope tag + polyA

    Plasmid
    #121751
    Purpose
    pMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    FLAG epitope tag + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121751-plasmid-map-sequence-id-237190

    Enlarge

  13. D5_Ld_280600

    Plasmid
    #135821
    Purpose
    Expression of Leishmania donovani ectodomain in mammalian cells
    Depositor
    Gavin Wright
    Article
    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.
    Insert
    major surface protease gp63, putative,leishmanolysin, putative
    Use
    Tags
    biotinylation sequence and His tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    135821-plasmid-map-sequence-id-274515

    Enlarge

  15. KZ401: pMVP (L3-L2) P2A-Neo + polyA

    Plasmid
    #121781
    Purpose
    pMVP L3-L2 entry plasmid, contains Neo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-Neo + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121781-plasmid-map-sequence-id-237277

    Enlarge

  17. KZ601: pMVP (L3-L2) P2A-Zeo + polyA

    Plasmid
    #121783
    Purpose
    pMVP L3-L2 entry plasmid, contains Zeo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-Zeo + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121783-plasmid-map-sequence-id-237280

    Enlarge

  18. KZ901: pMVP (L3-L2) P2A-eGFP + WPRE

    Plasmid
    #121772
    Purpose
    pMVP L3-L2 entry plasmid, contains eGFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-eGFP + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121772-plasmid-map-sequence-id-237267

    Enlarge

  26. KZ001: pMVP (L3-L2) P2A-eYFP + polyA

    Plasmid
    #121770
    Purpose
    pMVP L3-L2 entry plasmid, contains eYFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-eYFP + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121770-plasmid-map-sequence-id-237265

    Enlarge

  28. pDule-Acd82

    Plasmid
    #174099
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174099-plasmid-map-sequence-id-345716

    Enlarge

  29. LA601: pMVP (L3-L2) P2A-Puro + WPRE

    Plasmid
    #121785
    Purpose
    pMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectors
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-Puro + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121785-plasmid-map-sequence-id-237283

    Enlarge

  30. pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47452
    Purpose
    AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NI_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47452-plasmid-map-sequence-id-72981

    Enlarge

  31. pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47446
    Purpose
    AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5HD_C63)-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47446-plasmid-map-sequence-id-69438

    Enlarge

  32. pAAV_hSyn_TALEBB(NN)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47445
    Purpose
    AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NN_C63)-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47445-plasmid-map-sequence-id-72977

    Enlarge

  33. KZ501: pMVP (L3-L2) P2A-Puro + polyA

    Plasmid
    #121780
    Purpose
    pMVP L3-L2 entry plasmid, contains Puro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-Puro + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121780-plasmid-map-sequence-id-237276

    Enlarge

  35. KY901: pMVP (L3-L2) P2A-mCherry + polyA

    Plasmid
    #121766
    Purpose
    pMVP L3-L2 entry plasmid, contains mCherry-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-mCherry + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121766-plasmid-map-sequence-id-237260

    Enlarge

  36. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #48253
    Purpose
    LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    CRY2PHR-NLS-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha
    Available since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    48253-plasmid-map-sequence-id-70481

    Enlarge

  41. HD201: pMVP (L3-L2) IRES-eGFP + polyA

    Plasmid
    #121747
    Purpose
    pMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    IRES2-eGFP + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121747-plasmid-map-sequence-id-237185

    Enlarge

  43. LA001: pMVP (L3-L2) P2A-mCherry + WPRE

    Plasmid
    #121774
    Purpose
    pMVP L3-L2 entry plasmid, contains mCherry-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-mCherry + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121774-plasmid-map-sequence-id-237269

    Enlarge

  45. KA501: pMVP (L3-L2) pA; CMV::TETa-pA

    Plasmid
    #121801
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + CMV::TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121801-plasmid-map-sequence-id-237309

    Enlarge

  46. LA201: pMVP (L3-L2) P2A-eCFP + WPRE

    Plasmid
    #121776
    Purpose
    pMVP L3-L2 entry plasmid, contains eCFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-eCFP + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121776-plasmid-map-sequence-id-237272

    Enlarge

  50. pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47451
    Purpose
    AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NG_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47451-plasmid-map-sequence-id-72980

    Enlarge

Showing: 4551 - 4600 of 8813 results