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We narrowed to 5,133 results for: puc

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Showing: 4551 - 4600 of 5133 results

  3. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid
    #227306
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227306-plasmid-map-sequence-id-452076

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  6. AA145

    Plasmid
    #215937
    Purpose
    Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215937-plasmid-map-sequence-id-431395

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  7. kiCAP-AAV-M.Mus.2

    Plasmid
    #196682
    Purpose
    Rep/Cap plasmid for the production of M.Mus.2, an AAV capsid with CNS tropism in mice.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    REQQKLW insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196682-plasmid-map-sequence-id-386958

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  16. pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

    Plasmid
    #110830
    Purpose
    Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.
    Depositor
    Ande Satyanarayana
    Article
    Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:
    Insert
    AZGP1 (Azgp1 Mouse)
    Use
    Non-viral gene expression vector
    Tags
    T2A and dTomato
    Promoter
    CMV
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    110830-plasmid-map-sequence-id-214108

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  18. pSL0747 (pDonor_L-MmeI)

    Plasmid
    #130647
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (L*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATG --> TGTTGGAG
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130647-plasmid-map-sequence-id-255647

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  19. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid
    #207786
    Purpose
    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207786-plasmid-map-sequence-id-413796

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  22. pSL0746 (pDonor_R-MmeI)

    Plasmid
    #130646
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (R*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATA --> TGTTGGAA
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130646-plasmid-map-sequence-id-255642

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  26. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid
    #207760
    Purpose
    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207760-plasmid-map-sequence-id-413789

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  30. kiCAP-AAV-PAL2

    Plasmid
    #196691
    Purpose
    Rep/Cap plasmid for the production of PAL2, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    PTQGTVR insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196691-plasmid-map-sequence-id-388689

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  31. ORCO-T2A-QF2

    Plasmid
    #162524
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162524-plasmid-map-sequence-id-354668

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  41. Gr4-T2A-QF2 HDR plasmid

    Plasmid
    #140944
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140944-plasmid-map-sequence-id-313602

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Showing: 4551 - 4600 of 5133 results