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Showing: 1821 - 1840 of 7015 results

  1. pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

    Plasmid
    #179429
    Purpose
    Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-5kb-mch
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179429-plasmid-map-sequence-id-355448

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  6. pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113701
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113701-plasmid-map-sequence-id-232036

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  7. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161928
    Purpose
    Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    Surface marker and Citrine activation reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161928-plasmid-map-sequence-id-325287

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  8. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161927
    Purpose
    Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    Surface marker and Citrine repression reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    161927-plasmid-map-sequence-id-324349

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  9. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119946
    Purpose
    Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    119946-plasmid-map-sequence-id-237566

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  10. pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #188747
    Purpose
    Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Surface marker and mCitrine reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188747-plasmid-map-sequence-id-371444

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Showing: 1821 - 1840 of 7015 results