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20,415 results

Showing: 4851 - 4900 of 20415 results

  1. AAV-hSyn-RiboL1-jGCaMP8m

    Plasmid
    #169248
    Purpose
    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Insert
    RiboL1-jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    169248-plasmid-map-sequence-id-349877

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  9. pRVL-2

    Plasmid
    #104580
    Purpose
    Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Mouse IgG2c CH1/CH2/CH3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104580-plasmid-map-sequence-id-469485

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  10. Artichoke

    Plasmid
    #73320
    Purpose
    Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Article
    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and EF1a
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    73320-plasmid-map-sequence-id-135028

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  11. pBiFC-VN173

    Plasmid
    #22010
    Purpose
    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays
    Depositor
    Chang-Deng Hu
    Article
    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.
    Insert
    VN173
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Venus residus 1-172
    Promoter
    Available since
    Sept. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    22010-plasmid-map-sequence-id-354487

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  19. ADRB3-Tango

    Plasmid
    #66221
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRB3 (ADRB3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  25. 5UAS zfSynaptophysin:GFP-5UAS DsRedExpress

    Plasmid
    #74317
    Purpose
    A fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafish
    Depositor
    Stephen Smith, Martin Meyer
    Article
    Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.
    Inserts
    synaptophysin
    DsRedExpress
    Use
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    5UAS
    Available since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    74317-plasmid-map-sequence-id-314170

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  26. ERM-APEX2

    Plasmid
    #79055
    Purpose
    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    APEX2 anchored to the cytosolic face of the ER membrane
    Use
    Lentiviral
    Tags
    Residues 1-27 of P450 oxidase 2C1 and V5
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV immearly promotor
    Available since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79055-plasmid-map-sequence-id-419573

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  28. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid
    #62723
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV1 and AAV5
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available since
    April 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62723-plasmid-map-sequence-id-166279

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  31. HR_G3BP1-V5-APEX2-GFP

    Plasmid
    #105284
    Purpose
    homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus
    Depositor
    Eugene Yeo
    Article
    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do
    Insert
    G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)
    Use
    Human homologous recombination donor
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105284-plasmid-map-sequence-id-229022

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  32. pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) (AAV5)

    Viral Prep
    #121538-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) (#121538). In addition to the viral particles, you will also receive purified pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) plasmid DNA. Synapsin-driven, HA-tagged hM4D(Gi) for neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Christopher Richie
    Article
    Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111
    Promoter
    Syn
    Tags
    HA
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only

  37. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid
    #72325
    Purpose
    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-mEGFP-SpyTag
    Use
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72325-plasmid-map-sequence-id-137259

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  39. pExp-GB1

    Plasmid
    #112565
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-GB1 and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112565-plasmid-map-sequence-id-217228

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  41. pLentiV2-dCas9-VP64

    Plasmid
    #141104
    Purpose
    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
    Depositor
    Igor Ulitsky
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141104-plasmid-map-sequence-id-298612

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  43. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV9)

    Viral Prep
    #162380-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    CAG
    Tags
    None
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  45. pJYDN

    Plasmid
    #162450
    Purpose
    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162450-plasmid-map-sequence-id-321442

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  46. pJR103

    Plasmid
    #187242
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187242-plasmid-map-sequence-id-374822

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  47. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (AAV2)

    Viral Prep
    #50467-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (#50467). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine plasmid DNA. CaMKIIa-driven HA-tagged hM4D(Gi) and bicistronic mCitrine expression, for chemogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    mCitrine
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 4851 - 4900 of 20415 results