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169,450 results

Showing: 1 - 50 of 169450 results

  1. psPAX2

    Plasmid
    #12260
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Lentiviral; Packaging
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12260-plasmid-map-sequence-id-467229

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  2. pMD2.G

    Plasmid
    #12259
    Purpose
    VSV-G envelope expressing plasmid
    Depositor
    Didier Trono
    Insert
    VSV G
    Use
    Lentiviral; Envelope
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12259-plasmid-map-sequence-id-443804

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  3. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV Retrograde)

    Viral Prep
    #87306-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Li Zhang
    Article
    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.
    Promoter
    EF1a
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only

  4. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (AAV8)

    Viral Prep
    #154868-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available Since
    Aug. 19, 2022
    Availability
    Academic Institutions and Nonprofits only

  5. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV1)

    Viral Prep
    #105553-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV Retrograde)

    Viral Prep
    #105553-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. pMDLg/pRRE

    Plasmid
    #12251
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    HIV-1 GAG/POL
    Use
    Lentiviral; Packaging
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12251-plasmid-map-sequence-id-433915

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  8. pRSV-Rev

    Plasmid
    #12253
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    Rev (rev )
    Use
    Lentiviral; Packaging
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12253-plasmid-map-sequence-id-443803

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  9. pAAV2/9n

    Plasmid
    #112865
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap9
    Use
    AAV
    Promoter
    Truncated P5
    Available Since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112865-plasmid-map-sequence-id-350300

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  10. lentiCas9-Blast (Lentiviral Prep)

    Viral Prep
    #52962-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Promoter
    EFS-NS
    Available Since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100853-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available Since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  17. pCMV-VSV-G

    Plasmid
    #8454
    Purpose
    Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    8454-plasmid-map-sequence-id-404044

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  19. lentiCas9-Blast

    Plasmid
    #52962
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Lentiviral Prep
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52962-plasmid-map-sequence-id-322376

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  21. pAAV-hSyn-hM4D(Gi)-mCherry (AAV9)

    Viral Prep
    #50475-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  22. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid
    #71294
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Article
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    GNSTM glycosylation motif, HA, Lamp2 signal pepti…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71294-plasmid-map-sequence-id-418480

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  24. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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  26. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV8)

    Viral Prep
    #44361-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available Since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. pAAV-S5E2-GFP-fGFP (AAV PHP.eB)

    Viral Prep
    #135631-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-GFP-fGFP (#135631). In addition to the viral particles, you will also receive purified pAAV-S5E2-GFP-fGFP plasmid DNA. Bicistronic expression of GFP and membrane-targeted GFP under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Article
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Tags
    EGFP
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only

  29. AAV.TBG.PI.Cre.rBG (AAV8)

    Viral Prep
    #107787-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from AAV.TBG.PI.Cre.rBG (#107787). In addition to the viral particles, you will also receive purified AAV.TBG.PI.Cre.rBG plasmid DNA. TBG-driven expression of Cre. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    TBG
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  34. pAAV2/5

    Plasmid
    #232922
    Purpose
    AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Rep2/Cap5
    Use
    AAV
    Expression
    Mammalian
    Available Since
    Sept. 17, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232922-plasmid-map-sequence-id-458562

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  35. pAAV-hSyn-DIO-mCherry (AAV8)

    Viral Prep
    #50459-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available Since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  39. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (AAV9)

    Viral Prep
    #100834-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (#100834). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 plasmid DNA. CamKII-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CaMKII
    Available Since
    April 25, 2018
    Availability
    Academic Institutions and Nonprofits only

  41. pcDNA3-EGFP

    Plasmid
    #13031
    Purpose
    Mammalian expression vector for cloning your gene fused to EGFP
    Depositor
    Doug Golenbock
    Insert
    Enhanced Green Fluorescent Protein
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13031-plasmid-map-sequence-id-305137

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  42. AAV pCAG-FLEX-EGFP-WPRE (AAV9)

    Viral Prep
    #51502-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do
    Promoter
    CAG
    Tags
    EGFP (Cre-dependent)
    Available Since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only

  43. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106280-plasmid-map-sequence-id-354474

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  47. pENN.AAV.CMVs.Pl.Cre.rBG (AAV9)

    Viral Prep
    #105537-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Available Since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  48. pAAV-EF1a-Flpo (AAV1)

    Viral Prep
    #55637-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Available Since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only

  49. lenti dCAS-VP64_Blast

    Plasmid
    #61425
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Lentiviral Prep
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    61425-plasmid-map-sequence-id-457486

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Showing: 1 - 50 of 169450 results