159,909 results

1. pET-28c S2

   Plasmid

   #171920

   Purpose

   Express RNA-based (p)ppGpp sensor

   Depositor

   Mingxu You

   Article

   Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

   Insert

   RNA-based (p)ppGpp sensor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-M-Flag-BAP1-C91A

   Plasmid

   #125841

   Purpose

   express mutant BAP1-C91A

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   C91A

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-DsRed_IRES_EGFP

   Plasmid

   #92194

   Purpose

   Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   DsRed-IRES-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-pmExRai-AKAR2

   Plasmid

   #161755

   Purpose

   Plasma membrane-targeted ExRai-AKAR2.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   Lyn-ExRai-AKAR2
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT374

   Plasmid

   #125615

   Purpose

   Expresses evoFERNY-BE4max-NG in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoFERNY-BE4max-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H102P D104N

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEGFP-N1

   Plasmid

   #54767

   Purpose

   Localization: N1 Cloning Vector, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pCDNA3.1-Nanog

   Plasmid

   #28221

   Depositor

   Linzhao Cheng

   Article

   Chou et al Cell Res. 2011 Jan 18. ():.

   Insert

   Nanog (NANOG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pANAP

   Plasmid

   #48696

   Purpose

   Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

   Depositor

   Peter Schultz

   Article

   Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

   Inserts

   8 copies of the amber suppressor tRNACUA-Leu
   AnapRS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutant E.coli leucyl synthetase specific for Anap…

   Promoter

   CMV and human H1 promoter

   Available since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG654 pFAST

   Plasmid

   #172861

   Purpose

   Expresses pFAST in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   pFAST
   

   Use

   Tags

   Myc tag (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-NLS-Cas9-6xHis

    Plasmid

    #62934

    Purpose

    Expression of NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    NLS-Cas9-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPE4max-Blasticidin

    Plasmid

    #194905

    Purpose

    Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.

    Depositor

    George Church

    Article

    Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:

    Insert

    BSD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid

    #218190

    Purpose

    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    His and NSP4 signal secretion peptide

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. amilCP chromoprotein

    Plasmid

    #117847

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. 8xNFAT-ZsG-hCD4

    Plasmid

    #162746

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with human CD4 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

    Insert

    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXL010-Wnt dual (GFP-Fire) reporter

    Plasmid

    #40588

    Depositor

    Sean Palecek

    Article

    Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

    Insert

    Wnt reporter sequences
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Wnt promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid

    #170186

    Purpose

    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

    Depositor

    Bradley Bernstein

    Article

    Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

    Insert

    CreERT2-T2A-eBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti-sgRNA puro

    Plasmid

    #104990

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFPC1-FLAG-Synaptojanin 1-145

    Plasmid

    #22293

    Depositor

    Pietro De Camilli

    Article

    Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

    Insert

    Synaptojanin 1_145 (SYNJ1 Human)
    

    Use

    Tags

    Flag and GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMMS22L-siR3

    Plasmid

    #221013

    Purpose

    mammalian expression vector of myc-Flag tagged MMS22L siRNA resistant

    Depositor

    Daniel Durocher

    Article

    O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

    Insert

    MMS22L (MMS22L Human)
    

    Use

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    silent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…

    Promoter

    CMV

    Available since

    July 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pJW2098

    Plasmid

    #163094

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNTI647 dCas9-Mxi1 TetR KanMX

    Plasmid

    #139474

    Purpose

    Integrates CRISPRi effector and tet-responsive regulator

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Inserts

    dCas9-Mxi1
    Tet Repressor
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1 and pTEF1

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pmCherry-RPA32

    Plasmid

    #211382

    Purpose

    mammalian expression of human RPA32 N-terminally fused to mCherry

    Depositor

    Helmut Pospiech

    Insert

    RPA32 (RPA2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7II-2N4Rtau

    Plasmid

    #177653

    Purpose

    Recombinant protein synthesis of human Tau

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    Tau (2N4R) (MAPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. N-Cadherin-EGFP

    Plasmid

    #18870

    Depositor

    Valeri Vasioukhin

    Article

    Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

    Insert

    N-Cadherin (Cdh2 Mouse)
    

    Use

    Tags

    EGFP, Kanamycin, and N-Cadherin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMK425 (GAL-OsTIR1(F74A))

    Plasmid

    #140657

    Purpose

    GAL-OsTIR1(F74A)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    GAL-OsTIR1(F74A)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDR195

    Plasmid

    #36028

    Depositor

    Dominique Loque, Wolf Frommer

    Article

    Rentsch et al FEBS Lett. 1995 Aug 21;370(3):264-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEB-Tg

    Plasmid

    #28220

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Jan 18. ():.

    Insert

    SV40 large T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3/Flag-METTL3

    Plasmid

    #53739

    Purpose

    For expression of METTL3 (methyltransferase-like 3) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Methyltransferase-like 3 (METTL3 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 7TGP

    Plasmid

    #24305

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-eGFP // SV40-PuroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AiP14533-pAAV-DLX2.0-minBG-DIO-SYFP2-WPRE-HGHpA (Alias: CN4533)

    Plasmid

    #222938

    Purpose

    Cre-dependent expression of SYFP2 under the DLX2.0 enhancer

    Depositor

    Jonathan Ting

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge