167,150 results
-
Plasmid#213864PurposeBinary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.DepositorInsertchloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)
ExpressionBacterial and PlantAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2466
Plasmid#142094PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-Neo-rTOM20-TagBFP
Plasmid#210332PurposeOverexpression of rTOM20DepositorAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCS109
Plasmid#209330PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mTagBFP2 fusion protein.DepositorTypeEmpty backboneExpressionPlantAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pATT-BsaI-Pmag-NmagHigh1
Plasmid#202713PurposeContains Magnet flanked by linkers and BsaI sites insert for transposon insertion libraries.DepositorInsertlinker-pMag-RBS-nMagHigh1-linker
UseStaging plasmid to make transposon insertion libr…Available SinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0733
Plasmid#141676PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_1-180_AE
Plasmid#148578PurposeMammalian Expression of HsGIGYF2_1-180DepositorInsertHsGIGYF2_1-180 (GIGYF2 Human)
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC34A2_STOP
Plasmid#161254PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC34A2 (SLC34A2 Human)
ExpressionMammalianAvailable SinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC34A3_STOP
Plasmid#161242PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC34A3 (SLC34A3 Human)
ExpressionMammalianAvailable SinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC20A2_STOP
Plasmid#161188PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC20A2 (SLC20A2 Human)
ExpressionMammalianAvailable SinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
tubulin-OeNL_pcDNA3
Plasmid#89523PurposeOrange color variant of bright luminescent protein, fused to b-tubulinDepositorInsertOeNL
Tagsb-tubulinExpressionMammalianPromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRGEB31
Plasmid#51295PurposeBinary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.DepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRice snoRNA U3 and dual 35S promoterAvailable SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG
Plasmid#222852PurposeMammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 FcDepositorInsertAT118-L
TagsIgG1_FcExpressionMammalianMutationL234A, L235A, P329GAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKIKOarsBKm
Plasmid#46766PurposeE.coli genomic integration at arsBDepositorTypeEmpty backboneUseE.coli genomic integrationAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS aP2 promoter (5.4kb) polyA
Plasmid#11424DepositorInsertaP2 promoter (Fabp4 Mouse)
ExpressionBacterialAvailable SinceApril 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-SUMO-TEV-TtCsm6
Plasmid#172486PurposeBacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.DepositorInsertT. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
TagsHis-SUMO-TEVExpressionBacterialPromoterT7 promoter and lac operatorAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_FLAG-HUWE1
Plasmid#187121Purposemammalian cell expression of FLAG-HUWE1DepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya
Plasmid#186980Purposemammalian expression of Cluc-Gluc reporterDepositorInsertCluc, Gluc
UseLuciferaseAvailable SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 puro - CRBN (#1)
Plasmid#166240PurposesgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1DepositorAvailable SinceDec. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiSAM v2 (Puro)
Plasmid#92062PurposeModified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 (sgRNA) and EF1a (dCas9-VP64)Available SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)
Plasmid#163056Purposemito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)DepositorInsertmito-roGFP2-Grx1
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV
Plasmid#208635Purposefor E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.DepositorTypeEmpty backboneTags6xhisExpressionBacterialPromoterT7Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A2
Plasmid#131964PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A2 (SLC22A2 Human)
ExpressionMammalianAvailable SinceOct. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYTK072
Plasmid#65179PurposeEncodes ConRE as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConRE
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-cfGFP
Plasmid#200773PurposeExpresses cysteine-free GFP (cfGFP) under synapsin promoterDepositorHas ServiceAAV8Insertcysteine-free GFP
UseAAVPromoterSynapsinAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
Tags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8f-WPRE
Plasmid#162376PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8f
UseAAVTags6xHisExpressionMammalianMutationQ315LPromoterSynapsinAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pELPS-eDHFR-YFP-T2A-Renilla
Plasmid#193253PurposeTriple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferaseDepositorInsertThe eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
UseLentiviralTagsRenilla luciferase (rLuc) and yellow fluorescent …MutationNonePromoterEF1aAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-tdTomato-P2A-BlasR (LRT2B)
Plasmid#110854PurposeLentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markersDepositorInsertsgRNA scaffold with spacer
UseLentiviralMutationWTPromoterU6Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-SS-antiFLAGscFv-TM
Plasmid#198303PurposeDisplays anti-FLAG scFv from the surface of mammalian cellsDepositorInsertpHCMV-SS-antiFLAGscFv-TM
ExpressionMammalianAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only