168,165 results

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pAAV-DIO-COX4-dAPEX2

Plasmid

#117177

Purpose

Plasmid name in publication: pAAV-DIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

COX4-dAPEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBEVY-L

Plasmid

#51226

Purpose

bi-directional expression vector for yeast, constitutively active, LEU2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GPD/ADH1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV6-Neo-ISG15-LRAA

Plasmid

#80405

Purpose

overexpression of nonconjugative ISG15

Depositor

Isidoro Martinez

Article

Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

Insert

ISG15 (ISG15 Human)

Expression

Mammalian

Mutation

nonconjugative ISG15, LRAA

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pProm1_BCD1-GFP

Plasmid

#80649

Purpose

Promoter1 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-NRASG12D-BFP

Plasmid

#236202

Purpose

Expresses NRASG12D in mammalian cells

Depositor

Michael Heuser

Article

Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

Insert

NRAS (NRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12D

Promoter

MSCV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLVUHshp53-tTR-KRAB

Plasmid

#11654

Purpose

Tet-regulated (Tet-on) lentiviral vector for shp53 (hUbiquitin promoter) - 3rd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO (TP53 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFR-PATagRFP

Plasmid

#31950

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

EGF receptor (EGFR)

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available Since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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#2.4 pre-G-SynapShot-TagBFP

Plasmid

#214721

Purpose

Expresses green #2.4 pre-SynapShot and TagBFP

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#2.4 pre-G-SynapShot

Use

AAV

Tags

TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKCE

Plasmid

#23551

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCE (PRKCE Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pTrEno

Plasmid

#115474

Purpose

Eno-promoter driven expression vector for protein production in Trichoderma reesei

Depositor

Steve Decker

Article

Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d

Insert

Cel7A

Use

Unspecified

Mutation

Introns have been removed

Promoter

Trichoderma reesei Enolase (Eno) promoter

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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GADD45 WT-luc

Plasmid

#8356

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

GADD45 promoter WT (GADD45A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pREDawn-AmpR-MCS

Plasmid

#188978

Purpose

For red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Pan-Neurofascin (extracellular) scFv [A12/18]

Plasmid

#190485

Purpose

Mammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKT-PH-GFP

Plasmid

#18836

Depositor

Craig Montell

Article

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Insert

AKT-PH domain (AKT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains amino acids 1-148 (compared to GenBank r…

Promoter

CMV

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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CDK2 gRNA (BRDN0001148866)

Plasmid

#77191

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK2 (CDK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJC005.gent

Plasmid

#182739

Purpose

CRISPR-Cas12a genome editing of E. faecium

Depositor

James Collins

Article

Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

Type

Empty backbone

Use

CRISPR

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-mStayGold-CENPA

Plasmid

#227283

Purpose

Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

CENPA Homology Arms flanking a mStayGold Tag (CENPA Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-CENPA

Plasmid

#227282

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CENPA (CENPA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE136

Plasmid

#118490

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS2-puro

Plasmid

#158457

Purpose

Constitutive lentiviral expression of human TMPRSS2.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 nonmutant

Plasmid

#183813

Purpose

Express CD74-ROS1 fusion (C6;R34) in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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CDK1as_T2A_puromycin

Plasmid

#118595

Purpose

One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

CDK1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Stat3 pcDNA3

Plasmid

#8706

Purpose

See Depositor Comments below for variant information.

Depositor

Jim Darnell

Article

Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

Insert

Stat3 (Stat3 Mouse)

Expression

Mammalian

Mutation

* see comments

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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CIp10 - γmGFP (γmGFP based)

Plasmid

#163119

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

gamma mGFP

Use

Candida albicans expression

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-14-3-3 epsilon GST

Plasmid

#13279

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 epsilon (Ywhae Rat)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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vHB8

Plasmid

#193454

Purpose

Expression of GFP-Cas9-NLS and gRNA in A. castellanii

Depositor

Chantal Abergel

Article

Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

Insert

GFP-Cas9-NLS

Use

CRISPR

Tags

GFP

Promoter

GAPDH

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Sec61b-C1

Plasmid

#90994

Purpose

Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mCherry-Sec61b (SEC61B Human, Synthetic)

Tags

mCherry (red fluorescence)

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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DYRK1A_pCSdest

Plasmid

#53791

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

DYRK1A (DYRK1A Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSLQ10844

Plasmid

#183956

Purpose

CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

HyperdCas12a

Use

CRISPR

Tags

HA, Hygromycin Resistance, and mCherry

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a-hPKM2

Plasmid

#44242

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

Insert

Pyruvate Kinase M2 (PKM Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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