168,189 results

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pET-11b_PcrA_M6_H93A

Plasmid

#194156

Purpose

Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

Depositor

Taekjip Ha

Article

Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

Insert

Engineered helicase PcrA_M6

Tags

6x His tag and FLAG tag

Expression

Bacterial

Mutation

H93A, C96A, N187C, C247A, L384V, L409C

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJL-TRBO

Plasmid

#80082

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.

Depositor

John Lindbo

Article

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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HP-Cas9hGem-Ade2-LEU2-LINEAR

Plasmid

#174839

Purpose

pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TDH3p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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LV-AR-KKKAAA

Plasmid

#171219

Purpose

Lenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.

Depositor

Steven Balk

Article

Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

Insert

AR-KKKAAA (AR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

AR-K630A-K632A-K633A

Promoter

EF-1a

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymNeongreen-URA3

Plasmid

#168059

Purpose

Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Tags

ymNeonGreen

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-Nucleocapsid

Plasmid

#168967

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-Nucleocapsid (N )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-Nucleocapsid

Plasmid

#168947

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-Nucleocapsid (EYW02_gp8 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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PCMV-flag-Caspase11

Plasmid

#21145

Depositor

Junying Yuan

Article

Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

Insert

Caspase11 (Casp4 Mouse)

Tags

flag

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

Plasmid

#189752

Purpose

Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mTurquoise2_spatial-linker-P2A_mScarlet3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

Plasmid

#170712

Purpose

dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Inserts

mNeonGreen
mScarlet

Use

Lentiviral

Expression

Mammalian

Promoter

TNNT2 and aMHC

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_VHL_WT

Plasmid

#81874

Purpose

Gateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF2857

Plasmid

#143642

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ID4 (ID4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pUltra-Chili-Luc

Plasmid

#48688

Purpose

3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferase

Depositor

Malcolm Moore

Article

Fujiwara et al Mol Cancer Ther. 2021 Jun 4. pii: 1535-7

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

Plasmid

#187956

Purpose

FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-hHDAC4-SpdCas9-tagBFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_Zika_NS3_Flag

Plasmid

#79635

Purpose

Third generation Self-inactivating lentivector expressing NS3 from Zika virus

Depositor

Vaithi Arumugaswami

Insert

NS3

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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iRFP-P4M-SidM

Plasmid

#51470

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Insert

P4M-SidM(546-647)

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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PCE(cm24Cre)

Plasmid

#242425

Purpose

for chromosome editing

Depositor

Caixia Gao

Article

Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

Insert

PE, cm24Cre

Use

CRISPR

Expression

Plant

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCS Omomyc

Plasmid

#113164

Purpose

Omomyc expression in mammalian cells

Depositor

Sergio Nasi

Article

Soucek et al Oncogene. 1998 Nov 12;17(19):2463-72. do

Insert

Omomyc (MYC Human)

Expression

Mammalian

Mutation

E57T, E64I, R70Q, R71N

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab5a-7

Plasmid

#55126

Purpose

Localization: Ras GTPase, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Rab5a

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pMarsL274G

Plasmid

#63177

Purpose

This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.

Depositor

David Tirrell

Article

Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

Insert

MarsL274G (Mars1 Mouse)

Expression

Mammalian

Mutation

L274G

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR

Plasmid

#109055

Purpose

Bacterial expression of E.coli DHFR protein

Depositor

Stephen Benkovic

Article

Cameron et al Biochemistry. 1997 Dec 16;36(50):15792-8

Insert

ecDHFR

Expression

Bacterial

Promoter

T7

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP HA

Plasmid

#59759

Purpose

encodes multiple copies of an epitope tag HA

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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FUGW-Golgi-TurboID

Plasmid

#200964

Purpose

3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Golgi-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+-H2B-pr-mEosFP

Plasmid

#141101

Purpose

H2B fused to pr-mEosFP for chromatin visualization and primed conversion

Depositor

Periklis Pantazis

Article

Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

Insert

Histone 2B

Tags

pr-mEosFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBXNH3

Plasmid

#47067

Purpose

FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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PINK1 gRNA (BRDN0001145357)

Plasmid

#78036

Purpose

3rd generation lentiviral gRNA plasmid targeting human PINK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PINK1 (PINK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pKOR1

Plasmid

#133446

Purpose

Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection

Depositor

Taeok Bae, Olaf Schneewind

Article

Bae et al Plasmid. 2006 Jan;55(1):58-63. doi: 10.1

Type

Empty backbone

Use

E.coli/s.aureus shuttle vector

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR1A-Huwe1

Plasmid

#37431

Depositor

Jean Cook

Insert

Huwe1 (HUWE1 Human)

Use

Gateway entry

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pHM106Env

Plasmid

#139024

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein (env HIV-1)

Expression

Mammalian

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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