159,258 results

1. pcDNA4-Flag-XPA

   Plasmid

   #22895

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   XPA (XPA Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

2. EGFP-Rab13-T22N

   Plasmid

   #110501

   Purpose

   Dominant Negative

   Depositor

   Bernardo Mainou

   Article

   Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

   Insert

   Rab13-T22N (RAB13 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T22N

   Promoter

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV-actin prom-dsRed-adra2a.1227.shRNA

   Plasmid

   #67880

   Purpose

   expresses dsRed and shRNA to knock down adra2a receptors

   Depositor

   William Wisden

   Article

   Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

   Insert

   dsRed and shRNA to knock down the mouse adra2 receptor
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta actin

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

4. Cytoplasmic cpGFP

   Plasmid

   #215709

   Purpose

   Expresses cpGFP in the cytoplasm

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   cpGFP
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and cytoplasmic localization sequence

   Expression

   Mammalian

   Mutation

   circularly permuted GFP

   Promoter

   CMV

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330 Ctnnb1.1

   Plasmid

   #59911

   Purpose

   pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pmec-4::Chrimson::SL2::mCherry::unc-54

   Plasmid

   #107745

   Purpose

   Express Chrimson and mCherry in mechanosensory neurons.

   Depositor

   Andrew Leifer

   Article

   Liu et al Elife. 2018 Jun 26;7. pii: 36419. doi: 1

   Inserts

   Chrimson
   mCherry
   

   Use

   Tags

   Expression

   Insect and Worm

   Mutation

   Promoter

   mec-4

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. P2RY2-Tango

   Plasmid

   #66474

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   P2RY2 (P2RY2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. p5E-mitfa2.1

   Plasmid

   #81234

   Purpose

   zebrafish mitfa promoter entry vector for multisite Gateway cloning

   Depositor

   James Lister

   Insert

   mitfa promoter (mitfa Zebrafish)
   

   Use

   Multisite gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   mitfa, 2.1kb including 5' UTR

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-Rab13-Q67L

   Plasmid

   #110502

   Purpose

   Constitutive Active

   Depositor

   Bernardo Mainou

   Insert

   Rab13-Q67L (RAB13 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-N1.ARRDC1

    Plasmid

    #38320

    Depositor

    Quan Lu

    Article

    Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    ARRDC1 (ARRDC1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV_SEPT9_i1-msfGFP

    Plasmid

    #180328

    Purpose

    mammalian expression of human SEPT9_i1 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIRES2-mCherry-p53 deltaN

    Plasmid

    #49243

    Purpose

    expresses human p53 deltaN and mCherry in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53 deltaN (TP53 Human)
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    deltaN ( lacks 39 residues at the N-terminus)

    Promoter

    CMV

    Available since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVUHshp53-tTR-KRAB

    Plasmid

    #11654

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO (TP53 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. miniABEmax (pRZ900)

    Plasmid

    #131311

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUCmini-iCAP-AAV9-X1.1

    Plasmid

    #196836

    Purpose

    non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

    Insert

    Synthetic construct isolate AAV9-X1.1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ABE8.13-m

    Plasmid

    #136296

    Purpose

    expresses ABE8.13-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.13-m
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. gamma Synuclein-VC

    Plasmid

    #89734

    Purpose

    expresses human gamma Synuclein fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Eckermann et al Biochim Biophys Acta. 2015 Aug;1852(8):1

    Insert

    SNCG (SNCG Human)
    

    Use

    Tags

    C-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOTTC293 - pAAV EF1a V5-synuclein (WT)

    Plasmid

    #60057

    Purpose

    An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    alpha-synuclein (SNCA Human, human)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmEGFP-ADRA2A

    Plasmid

    #190753

    Purpose

    Expression of ADRA2A with mEGFP tag on N-terminus.

    Depositor

    Dave Piston

    Article

    Foust et al bioRxiv 2021.11.03.467169

    Insert

    ADRA2A (ADRA2A Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. (112) pcDNA3.1-GFP-Flag-Nup62-EPEA

    Plasmid

    #168101

    Purpose

    GFP and Flag tagged Nup62 with EPEA nanobody tag

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    GFP-Flag-Nup62-EPEA
    

    Use

    Tags

    EPEA, Flag, and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA4 CoV-2 nsp1 3xFLAG

    Plasmid

    #176057

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-CMV-PdCO-mScarlet

    Plasmid

    #198507

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HsGRK6-mVenus

    Plasmid

    #137780

    Purpose

    Visualization of GRK6

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Human GRK6 (GRK6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENTR U6-hTERTsh

    Plasmid

    #111385

    Purpose

    Knock down of hTERT transcript

    Depositor

    Klaus Holzmann

    Article

    Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

    Insert

    shRNA C3 against hTERT
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid

    #220612

    Purpose

    Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-ArgiNLS-AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. gag-P3-pol

    Plasmid

    #211374

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-P3-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFA6a-link-ymScarletI-SpHis5

    Plasmid

    #219844

    Purpose

    tagging vector to tag genes with ymScarletI and a HIS5 gene

    Depositor

    Bas Teusink

    Insert

    ymScarletI-SpHis5
    

    Use

    Tags

    ymScarletI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pEGFP-C1-TULP3

    Plasmid

    #218741

    Purpose

    Expresses C-terminally EGFP-tagged TULP3 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    TULP3 (TULP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EC67908

    Plasmid

    #211795

    Purpose

    Over expresses Cas12a and GR-GRF-GIF. Must receive guide cassettes

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    HptII, Cas12a, GR-GRF-GIF
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDIV270

    Plasmid

    #226181

    Purpose

    Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF1p (Komagataella phaffii)

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge