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159,258 results

Showing: 2041 - 2070 of 159258 results

  1. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  6. scFLARE1-p2A-eGFP

    Plasmid
    #158699
    Purpose
    Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker
    Depositor
    Alice Ting
    Article
    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p
    Insert
    scFLARE1-p2A-eGFP
    Use
    AAV
    Tags
    Flag, V5. eGFP
    Expression
    Mammalian
    Mutation
    Truncated form of TEV protease (220-242 amino aci…
    Promoter
    CMV
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158699-plasmid-map-sequence-id-315073

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  15. Lamp1_mStayGold

    Plasmid
    #221902
    Purpose
    Expresses mStayGold fused to Lamp1
    Depositor
    Janelia Integrative Imaging
    Insert
    Lamp1_mStayGold
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    221902-plasmid-map-sequence-id-438067

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  17. EB1-tdTomato

    Plasmid
    #50825
    Purpose
    expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs
    Depositor
    Erik Dent
    Insert
    EB1 (Mapre1 Mouse)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    50825-plasmid-map-sequence-id-377726

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  20. pLVX-puro-cOVA-IRES-BFP

    Plasmid
    #135074
    Purpose
    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.
    Depositor
    Maria Castro
    Insert
    cOVA-IRES-BFP (OVAL Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    135074-plasmid-map-sequence-id-266791

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  22. CXCR3-DuET

    Plasmid
    #213220
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR3 (CXCR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213220-plasmid-map-sequence-id-430782

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  23. mini-pHelper-1.0

    Plasmid
    #230933
    Purpose
    The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production
    Depositor
    Qizhao Wang
    Type
    Empty backbone
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230933-plasmid-map-sequence-id-458355

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  25. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid
    #174007
    Purpose
    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.
    Depositor
    Mark Histed
    Article
    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22
    Has Service
    AAV9
    Insert
    jGCaMP8s-P2A-ChrimsonR-ST
    Use
    AAV
    Tags
    6xHis and soma targeting motif
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    174007-plasmid-map-sequence-id-361587

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  30. pcDNA3/F-tractin=mStayGold

    Plasmid
    #212019
    Purpose
    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    F-tractin
    Use
    Tags
    mStayGold
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212019-plasmid-map-sequence-id-419223

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Showing: 2041 - 2070 of 159258 results