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pXJ-Myc-IRF1

Plasmid

#232641

Purpose

transient expresses IRF1 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

IRF1 (IRF1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-UPRE-luc2P-Hygro

Plasmid

#101788

Purpose

Luciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)

Depositor

Seiichi Oyadomari

Article

Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

Insert

3xUPRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-Gal3

Plasmid

#85662

Purpose

Fluorescent reporter for lysosomal damage

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

LGALS3 (LGALS3 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

Plasmid

#60910

Purpose

dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, and NLS

Expression

Mammalian

Promoter

dSV40 Promoter

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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AttB _EGFP

Plasmid

#171597

Purpose

Strong EGFP fluorescence from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Insert

EGFP

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pNP2916 [Cloning backbone, bRNA Landing Pad]

Plasmid

#247254

Purpose

Cloning backbone for cloning bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pNP3613 [Cloning backbone, recombinase]

Plasmid

#247264

Purpose

Cloning backbone for cloning bridge recombinase

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b-IPP1-His

Plasmid

#124137

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Inorganic pyrophosphatase (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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EMTB-mNeonGreen

Plasmid

#137802

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Ensconsin microtubule binding domain

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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cdk1 FRET sensor (2327)

Plasmid

#26064

Depositor

Jonathon Pines

Article

Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Insert

Cdk1 FRET Sensor

Tags

CFP variant, FLAG, and YFP variant

Expression

Mammalian

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pCBL101gg

Plasmid

#199735

Purpose

Golden Gate recipient vector for transgene overexpression

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

sacB

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

Plasmid

#161574

Purpose

Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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His-FGE

Plasmid

#75147

Purpose

Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

Depositor

Richard Fishel

Article

Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

Insert

FGE

Tags

Histidine tag and Thrombin

Expression

Bacterial

Mutation

N/A

Promoter

T7

Available Since

July 8, 2016

Availability

Academic Institutions and Nonprofits only
pET14b – E. coli Exonuclease I

Plasmid

#104552

Purpose

bacterial expression of Exonuclease I

Depositor

Charles Bell

Article

Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

Insert

Exonuclease I (sbcB E.coli)

Tags

6X-His tag followed by thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2 (Neo)

Plasmid

#89180

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEZT-BM

Plasmid

#74099

Purpose

Multi-purpose rapid titer bacmam expression vector

Depositor

Ryan Hibbs

Article

Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Bacmam, baculovirus

Tags

EGFP following p10 promoter

Expression

Mammalian

Promoter

CMV, p10

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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N8his-GFPenhancer-GGGGS4-LaG16

Plasmid

#140442

Purpose

We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

Depositor

Motoyuki Hattori

Article

Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

Insert

GFPenhancer-GGGGS4-LaG16

Tags

8 histidine tag

Expression

Bacterial

Promoter

T7 promotor

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiRhoA2G

Plasmid

#40179

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Use

Lentiviral

Tags

His-Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGreen_dualluc_3'UTR_sensor

Plasmid

#55206

Purpose

Dual-luciferase reporter for microRNA targeting in plants

Depositor

Michael Axtell

Article

Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

Inserts

Renilla luciferase
Firefly luciferase

Use

Luciferase; Plant transient expression

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

Plasmid

#217971

Purpose

Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.

Depositor

Robert Piper

Article

Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

Insert

2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4

Expression

Mammalian

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_501

Plasmid

#96922

Purpose

for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMIG-FLAG-MLL-AF9

Plasmid

#71443

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

Depositor

Daisuke Nakada

Article

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-Af9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-VP64_Blast

Plasmid

#192650

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48

Plasmid

#17605

Purpose

Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

Plasmid

#193465

Purpose

Dn29 Donor with promoter for mcherry and puro expression

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Dn29_attP

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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STAT3-GFP

Plasmid

#110495

Purpose

reporter of STAT3 signaling activity

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

4 repeats of STAT3 binding site M67 at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CRISPR

Plasmid

#160047

Purpose

For cloning sgRNA to knockout a specific gene in piggybac plasmid

Depositor

Xiaojun Lian

Article

Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

Type

Empty backbone

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EFS

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pQUAST-attB

Plasmid

#104880

Purpose

For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase system

Depositor

Christopher Potter

Type

Empty backbone

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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NOVA1 eGFP

Plasmid

#61275

Purpose

GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1

Depositor

Nicolas Charlet-Berguerand

Insert

NOVA1 (NOVA1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry

Plasmid

#193293

Purpose

episomal vector expressing mCherry fluorescent protein

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge