159,333 results

1. TRE-mCherry-p2A-bReaches-TS

   Plasmid

   #158701

   Purpose

   Reporter construct

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   mCherry-p2A-bReaches-TS
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ef1a_Large T-antigen_Ires_Puro

   Plasmid

   #18922

   Depositor

   Linzhao Cheng

   Article

   Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

   Insert

   Large T Antigen (SV40gp6 Simian virus 40)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-Blast-PIP-FUCCI

   Plasmid

   #138715

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PEmax-P2A-hMLH1dn

   Plasmid

   #174828

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-MAFB-IRF8-CEBPA

   Plasmid

   #190720

   Purpose

   For microglia differentiation from iPSC

   Depositor

   Li Gan

   Article

   Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

   Insert

   MAFB-IRF8-CEBPA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SLF063: Lenti-fCAG-jRGECO1a

   Plasmid

   #124775

   Purpose

   For general lentiviral or direct expression of cytosolic jRGECO1a

   Depositor

   Adam Cohen

   Article

   Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

   Insert

   jRGECO1a
   

   Use

   Lentiviral

   Tags

   NES-6xHIS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

7. pCDH-kz-CD20-Puro

   Plasmid

   #209759

   Purpose

   Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 APEX2-NES

   Plasmid

   #49386

   Purpose

   Expresses APEX2 in the cytosol of mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Flag - APEX2 - NES
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM-mTagBFP2

   Plasmid

   #228615

   Purpose

   Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_478

    Plasmid

    #179096

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (SpG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCB-2

    Plasmid

    #210388

    Purpose

    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

    Depositor

    Gilles van Wezel

    Article

    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

    Inserts

    dCas9
    CymR
    sgRNA cassette
    

    Use

    CRISPR; Integrative vector for use in streptomyces

    Tags

    Expression

    Mutation

    BsaI-flanked RFP cassette inserted in place of sp…

    Promoter

    SP11 and SP30

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF1-alpha-V

    Plasmid

    #27290

    Depositor

    Timothy Springer

    Article

    Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

    Insert

    integrin alpha V (ITGAV Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Isoform 2

    Promoter

    Available since

    March 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGP-CMV-jGCaMP8s

    Plasmid

    #162371

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

    Plasmid

    #196415

    Purpose

    Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smMyc
    

    Use

    AAV; Astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and GfaABC1D

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_PGK_SNIPR_Hinge Notch SNIPR (LaG17)

    Plasmid

    #188380

    Purpose

    Lentiviral vector - constitutive expression of an anti-GFP SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiGFP_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. FCHo2-pmCherryC1

    Plasmid

    #27686

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    FCHo2 (Fcho2 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)/AUG-DAP5-3XFLAG

    Plasmid

    #127331

    Purpose

    Express 3xFLAG tagged DAP5 protein from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    DAP5 (EIF4G2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-Con/Fon-oScarlet

    Plasmid

    #137136

    Purpose

    Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICH41331::pU6cm-pegR::EURb7

    Plasmid

    #227695

    Purpose

    Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    Altered epegRNA backbone with cloning sites for gRNA and RTT.
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6 composite

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiSAM v2 (Puro)

    Plasmid

    #92062

    Purpose

    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (sgRNA) and EF1a (dCas9-VP64)

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEGFP-C1-G3BP1-WT

    Plasmid

    #135997

    Purpose

    WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX601-mCherry

    Plasmid

    #84039

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with mCherry

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pU6-Cj-sgRNA

    Plasmid

    #89753

    Purpose

    U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    Cj-SgRNA
    

    Use

    Sgrna expression under u6 promoter

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. GLUT1-GFP

    Plasmid

    #89440

    Purpose

    GLUT1 construct

    Depositor

    Jeffrey Rathmell

    Article

    Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

    Insert

    GLUT1 (Slc2a1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AiP1653 - pAAV-AiE2333m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #214506

    Purpose

    AiE2333m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pYPQ230-RRV

    Plasmid

    #188543

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RRV
    

    Use

    CRISPR; Gateway compatible lbcas12a-rrv entry clo…

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, D156R, R182V

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pBAD-LacI

    Plasmid

    #46394

    Purpose

    Overproduction of LacI

    Depositor

    Florian Hollfelder

    Article

    Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

    Insert

    LacI
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    silent T to C mutation at position 19

    Promoter

    arabinose promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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28. pDule2-para-aminoPhe

    Plasmid

    #85503

    Purpose

    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

    Insert

    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32T, E107T, D158P, I159L, L162A

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. pMIG-hNFATc2

    Plasmid

    #74050

    Purpose

    retroviral expression plasmid for human NFATc2/C

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc2, isoform C (NFATC2 Human)
    

    Use

    Retroviral

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    silent mutation A1723C in the sequence of NM_1730…

    Promoter

    pMSCV-LTRs

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC18/promEco/Lysostaphin

    Plasmid

    #125765

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK-Sa1 promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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