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27,469 results

Showing: 5881 - 5900 of 27469 results

  2. pFD116

    Plasmid
    #124769
    Purpose
    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Publication
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Ptet
    dcas9
    PpflB sgRNA BsaI
    Use
    CRISPR
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124769-plasmid-map-sequence-id-246181

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  5. aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

    Plasmid
    #101299
    Purpose
    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.
    Depositor
    Alpha Yap
    Publication
    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-Catenin
    Expression
    Mammalian
    Mutation
    Contains amino acids 1-698 and 698-906 of alpha-C…
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101299-plasmid-map-sequence-id-246095

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  6. pcDNA3.1-CD40L-mgSrtA

    Plasmid
    #125795
    Purpose
    for co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.
    Depositor
    P. Chen
    Publication
    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD40L-mgSrtA
    Expression
    Mammalian
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    125795-plasmid-map-sequence-id-245283

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  13. CMV-CASANOVA

    Plasmid
    #113035
    Purpose
    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Publication
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113035-plasmid-map-sequence-id-224728

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  14. PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1

    Plasmid
    #85069
    Purpose
    Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)
    Depositor
    Dorus Gadella
    Publication
    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PH-PLCD1 (PLCD1 Human)
    Tags
    IRES-sYFP2-PH and mScarlet
    Expression
    Mammalian
    Mutation
    PLCD aa 1–170
    Promoter
    CMV
    Available since
    Oct. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85069-plasmid-map-sequence-id-269155

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  15. AICSDP-64: FUS-mEGFP

    Plasmid
    #114410
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FUS
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FUS Homology Arms with linker-mEGFP (FUS Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Sept. 5, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    114410-plasmid-map-sequence-id-220875

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  16. pEUI(+)

    Plasmid
    #111834
    Purpose
    For mamalian expression of Ecdysone receptor-based inducible transgene expression switch.
    Depositor
    Hyunju Ro
    Publication
    Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAL4-minimal VP16-EcR (EcR Synthetic)
    Expression
    Mammalian
    Promoter
    CMV, SV40, 10XUAS
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111834-plasmid-map-sequence-id-218213

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  20. pEGFP-Zdk1-EB1C

    Plasmid
    #107696
    Purpose
    C-terminal half of π-EB1, EGFP-tagged, dissociates from microtubule ends in blue light
    Depositor
    Torsten Wittmann
    Publication
    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPRE1 (MAPRE1 Human)
    Tags
    EGFP and Zdk1
    Expression
    Mammalian
    Mutation
    EB1 aa 186-268
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107696-plasmid-map-sequence-id-209382

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Showing: 5881 - 5900 of 27469 results