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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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27,467 results

Showing: 1301 - 1320 of 27467 results

  1. pMLM3705

    Plasmid
    #47754
    Purpose
    Expresses mammalian cell codon-optimized dCas9-VP64
    Depositor
    Keith Joung
    Publication
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    codon optimized dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9 (catalytically i…
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47754-plasmid-map-sequence-id-68222

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  9. pRK5-myc-Rac1-T17N

    Plasmid
    #12984
    Depositor
    Gary Bokoch
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rac1 DN (RAC1 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    T17N Dominant negative
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12984-plasmid-map-sequence-id-223318

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  13. pCMV-dCas13-M3nls

    Plasmid
    #155366
    Purpose
    Targeted m6A RNA methylation in mammalian cells
    Depositor
    David Liu
    Publication
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155366-plasmid-map-sequence-id-305808

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  14. CLYBL-TO-hNGN2-BSD-mApple

    Plasmid
    #124229
    Purpose
    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.
    Depositor
    Michael Ward
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NGN2 (NEUROG2 Human)
    Expression
    Mammalian
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124229-plasmid-map-sequence-id-476962

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  16. pEGFP-C1-AR V7

    Plasmid
    #86856
    Purpose
    Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)
    Depositor
    Michael Mancini, Marco Marcelli
    Publication
    Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Androgen receptor (AR) (AR Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Alternative splice variant 7 (alteration/deletion…
    Promoter
    CMV
    Available since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86856-plasmid-map-sequence-id-386709

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  18. HIP-flash (HOP-flash mutant)

    Plasmid
    #83466
    Purpose
    Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene
    Depositor
    Barry Gumbiner
    Publication
    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    Use
    Luciferase
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83466-plasmid-map-sequence-id-159299

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  20. EBFP2-H2B-6

    Plasmid
    #55243
    Purpose
    Localization: Nucleus/Histones, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B (H2BC11 Human)
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    55243-plasmid-map-sequence-id-159436

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Showing: 1301 - 1320 of 27467 results