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27,007 results

Showing: 2081 - 2100 of 27007 results

  5. pCMV-PE6a

    Plasmid
    #207851
    Purpose
    Mammalian expression of PE6a prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6a
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    207851-plasmid-map-sequence-id-450977

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  7. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid
    #158653
    Purpose
    making transgenics expressing a UAS driven mCherry and NTR 2.0
    Depositor
    Jeff Mumm
    Publication
    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
    Use
    Tol2-based transgenic expression in zebrafish/oth…
    Mutation
    F70A/F108Y
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    158653-plasmid-map-sequence-id-311196

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  8. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid
    #139988
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    139988-plasmid-map-sequence-id-275338

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  9. pX458M-53BP1-DN1S

    Plasmid
    #131045
    Purpose
    Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.
    Depositor
    Punam Malik
    Publication
    Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-53BP1-DN1S (TP53BP1 Human)
    Use
    CRISPR
    Tags
    GFP and SpCas9
    Expression
    Mammalian
    Mutation
    Only expressing residues 1231-1644 of complete 53…
    Promoter
    CBH
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    131045-plasmid-map-sequence-id-258265

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  10. pME-GAL4-ERT2-VP16

    Plasmid
    #82588
    Purpose
    Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression
    Depositor
    Kryn Stankunas
    Publication
    Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAL4-ERT2-VP16
    Promoter
    None
    Available since
    Dec. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    82588-plasmid-map-sequence-id-161151

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  12. pYFP-RPB1aAmr

    Plasmid
    #75284
    Purpose
    Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells
    Depositor
    Xavier Darzacq, Robert Singer
    Publication
    Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POLR2A (POLR2A Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    N792D alpha-amanitin–resistant
    Promoter
    CMV
    Available since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    75284-plasmid-map-sequence-id-496264

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  15. GFP_CD47_LU

    Plasmid
    #65474
    Purpose
    GFP tagged CD47 with its own long 3'UTR
    Depositor
    Christine Mayr
    Publication
    Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Signal peptide of CD47
    GFP
    CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
    Expression
    Mammalian
    Available since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65474-plasmid-map-sequence-id-117297

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  17. pTriEx4-H6-FGAm (FlincG3)

    Plasmid
    #49202
    Purpose
    Fluorescent reporter for cGMP
    Depositor
    John Garthwaite
    Publication
    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FlincG3
    Tags
    His tag + other tags
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Met to Lys in cpEGFP region similar to CGaMP3
    Promoter
    CMV
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    49202-plasmid-map-sequence-id-72703

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Showing: 2081 - 2100 of 27007 results