Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,870 results

Showing: 4161 - 4180 of 30870 results

  2. Strep-KIFC1*-mCh

    Plasmid
    #120169
    Purpose
    Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kinesin family member C1 (KIFC1 Human)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated KIFC1: 125-673 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120169-plasmid-map-sequence-id-251408

    Enlarge

  7. pEGFPC1-hVAP-A KD/MD

    Plasmid
    #104449
    Purpose
    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells
    Depositor
    Catherine Tomasetto
    Publication
    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    vesicle-associated membrane protein-associated protein A (VAPA Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    changed K94 to D and M96 to D
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104449-plasmid-map-sequence-id-201409

    Enlarge

  8. SUV[SET]-dCas9

    Plasmid
    #100088
    Purpose
    Catalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SUVAR39H1 (SUV39H1 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-SUV[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    deleted aa 1-76
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100088-plasmid-map-sequence-id-197559

    Enlarge

  9. AICSDP-13:FBL-mEGFP

    Plasmid
    #87427
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FBL Homology Arms with linker-mEGFP (FBL Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    87427-plasmid-map-sequence-id-173980

    Enlarge

  14. pKHR7

    Plasmid
    #74624
    Purpose
    a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.
    Depositor
    David Grunwald
    Publication
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cmlc2::mCherry
    Use
    Zebrafish expression
    Promoter
    cmlc2
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    74624-plasmid-map-sequence-id-204495

    Enlarge

  17. pEF_dCas9

    Plasmid
    #68416
    Purpose
    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68416-plasmid-map-sequence-id-120194

    Enlarge

  19. Beta-2-adrenergic receptor-YFP

    Plasmid
    #67146
    Purpose
    Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)
    Tags
    YFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67146-plasmid-map-sequence-id-121580

    Enlarge

  20. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Publication
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66080-plasmid-map-sequence-id-436984

    Enlarge

Showing: 4161 - 4180 of 30870 results