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30,881 results

Showing: 7401 - 7420 of 30881 results

  5. pAc5 HA3-eDHFR-T2A-neo

    Plasmid
    #86396
    Purpose
    for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection
    Depositor
    David Bentley
    Publication
    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HA3 eDHFR T2A neo
    Use
    CRISPR
    Tags
    HA3 eDHFR T2A neo
    Mutation
    R12Y, G67S and Y100I mutations in E coli DHFR
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86396-plasmid-map-sequence-id-170909

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  6. pTH1

    Plasmid
    #84451
    Purpose
    shuttle plasmid for sarA P1-dsRED expression
    Depositor
    Reindert Nijland
    Publication
    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DsRed.T3(DNT)
    Use
    Shuttle vector e.coli-s.aureus, expression of sgf…
    Available since
    Feb. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    84451-plasmid-map-sequence-id-163430

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  9. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid
    #84244
    Purpose
    Expresses GA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sa dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84244-plasmid-map-sequence-id-160887

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  10. pEGFP-Merlin S518D

    Plasmid
    #84295
    Purpose
    Expression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation site
    Depositor
    Jonathan Chernoff
    Publication
    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Merlin (NF2) (NF2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S518D
    Promoter
    CMV
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    84295-plasmid-map-sequence-id-163911

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  11. pITGA4-GFP

    Plasmid
    #81178
    Purpose
    Mammalian expression of human full length ITGA4-EGFP
    Depositor
    Chinten James Lim
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ITGA4 (ITGA4 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Contains KLGFFKR motif; A1002L (please see deposi…
    Promoter
    CMV
    Available since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    81178-plasmid-map-sequence-id-175302

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  12. pEGFP-Rab21-Q76L (CA)

    Plasmid
    #83422
    Purpose
    Mouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.
    Depositor
    Johanna Ivaska
    Publication
    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab21 (Rab21 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Residue 76 from Q to L (Q76L: CAG -> CTG)
    Promoter
    CMV
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83422-plasmid-map-sequence-id-159111

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  13. pICE-HA-MRE11-H63D

    Plasmid
    #82036
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRE11 (MRE11 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Exonuclease-dead mutant: H63D MRE11
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    82036-plasmid-map-sequence-id-155389

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  14. pMA-SpCas9-g4

    Plasmid
    #80787
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80787-plasmid-map-sequence-id-162407

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Showing: 7401 - 7420 of 30881 results