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27,462 results

Showing: 9461 - 9480 of 27462 results

  1. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Publication
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    229676-plasmid-map-sequence-id-457076

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  2. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

    Plasmid
    #229678
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Publication
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    monomeric StayGold, HA
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    229678-plasmid-map-sequence-id-457125

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  3. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  6. gag-TadCBEd

    Plasmid
    #225144
    Purpose
    Packaging plasmid for TadCBEd in v3 PE-eVLP architecture
    Depositor
    David Liu
    Publication
    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag-TadCBEd
    Expression
    Mammalian
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    225144-plasmid-map-sequence-id-445269

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  7. TDP-REGv1(mCherry-FLAG) in pTwist-CMV

    Plasmid
    #216165
    Purpose
    Expression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Publication
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry with C-terminal FLAG tag
    Expression
    Mammalian
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216165-plasmid-map-sequence-id-437747

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  9. SMN2E7-Fluc-Nluc-reporter

    Plasmid
    #221005
    Purpose
    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Publication
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fluc-SMN2E7-STOP-Nluc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221005-plasmid-map-sequence-id-437012

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  10. pDualIN-SMN2

    Plasmid
    #214430
    Purpose
    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7
    Depositor
    Chaolin Zhang
    Publication
    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMN2 EXON 7 (SMN2 Human)
    Expression
    Mammalian
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214430-plasmid-map-sequence-id-424358

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  12. ARTi

    Plasmid
    #214064
    Purpose
    Fluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR
    Depositor
    Sujatha Jagannathan
    Publication
    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboRFP
    Expression
    Mammalian
    Mutation
    Linker sequence present between RFP and GFP
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    214064-plasmid-map-sequence-id-423977

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  14. nLbCas12a

    Plasmid
    #213745
    Purpose
    For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nLbCas12a
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R, R1138A
    Promoter
    CMV
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213745-plasmid-map-sequence-id-422202

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  15. pmCer-DHX9

    Plasmid
    #211408
    Purpose
    mammalian expression of human DHX9 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DHX9 (DHX9 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211408-plasmid-map-sequence-id-420513

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  17. B-GRAM-W

    Plasmid
    #211707
    Purpose
    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells
    Depositor
    Yasunori Saheki
    Publication
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Tags
    ddFP-B3
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211707-plasmid-map-sequence-id-419219

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  18. pU6-PspCas13b-GEMSgRNA2

    Plasmid
    #204768
    Purpose
    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.
    Depositor
    Kate Meyer
    Publication
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    guide RNA targeting GEMS m6A sensor mRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204768-plasmid-map-sequence-id-408665

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Showing: 9461 - 9480 of 27462 results