30,695 results

1. pJK225

   Plasmid

   #71124

   Purpose

   Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coli

   Depositor

   T. J. Kappock

   Article

   Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

   Insert

   citrate synthase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. ClpP-his

   Plasmid

   #22144

   Depositor

   Robert Sauer

   Article

   Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

   Insert

   ClpP-His6
   

   Tags

   his

   Expression

   Bacterial

   Mutation

   C-terminal His tag

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDB179-DuraPETase

   Plasmid

   #218699

   Purpose

   Encodes codon optimized DuraPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   DuraPETase
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HotPETase_pET21b

   Plasmid

   #215407

   Purpose

   Expression construct for HotPETase gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   HotPETase
   

   Expression

   Bacterial

   Mutation

   S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKD4_mCherry

   Plasmid

   #187386

   Purpose

   To chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRT

   Depositor

   Roi Avraham

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJYS2_crtYf

   Plasmid

   #85544

   Purpose

   Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Insert

   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Expression

   Bacterial

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMG36E

   Plasmid

   #133853

   Purpose

   Expression vector

   Depositor

   Gerard Venema, Jan Kok

   Article

   van de Guchte et al Appl Environ Microbiol. 1989 Jan;55(1):2

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   P32

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBR1.cas9-NT

   Plasmid

   #197871

   Purpose

   Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

   Depositor

   Baojun Wang

   Article

   Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

   Inserts

   trancCRNA
   Cas9
   crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
   

   Expression

   Bacterial

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTAT-CRE

   Plasmid

   #35619

   Depositor

   Steven Dowdy

   Article

   Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

   Insert

   CRE (cre Bacteriophage P1)
   

   Tags

   6xHIS, T7 tag, and TAT

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pMLS7

    Plasmid

    #32056

    Purpose

    Vector optimized for constitutive gene expression in Burkholderia genus and related bacteria

    Depositor

    Miguel Valvano

    Article

    Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Ps7 promoter, originated from Paraburkholderia xe…

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a - Ec-ClpX

    Plasmid

    #111516

    Purpose

    Expresses E. coli ClpX with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    ClpX
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCasTet-λ

    Plasmid

    #128318

    Purpose

    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

    Depositor

    Christopher Johnston

    Article

    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

    Insert

    TetR and pTetR/TetO
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    Constitutive expression of cas9 and anhydro-tetra…

    Promoter

    tet promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid

    #181745

    Purpose

    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    human/zebrafish codon optimized SpCas9
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Keasling-2337

    Plasmid

    #100168

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    lacIq-Ptrc-ADS
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET Flag TEV LIC cloning vector (1L)

    Plasmid

    #29662

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    Flag and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE-Tn7-132

    Plasmid

    #118550

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRE-Tn7-135

    Plasmid

    #118553

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD-HisD-CreiLOV

    Plasmid

    #192817

    Purpose

    Expresses CreiLOV in bacteria

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    CreiLOV
    

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMal-Strep

    Plasmid

    #209345

    Purpose

    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagII

    Depositor

    Lucia Strader

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMAT11

    Plasmid

    #112592

    Purpose

    Used for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His-MBP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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