31,059 results

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pET28a-cMSP1E3D1

Plasmid

#199772

Purpose

Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

Insert

cMSP1E3D1

Tags

His6-Tag

Expression

Bacterial

Mutation

Extended cMSP1D1; external His-Tags flanking the …

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-60 Grx1-roGFP2-His

Plasmid

#64799

Purpose

Bacterial expression of His-tagged Grx1-roGFP2

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Tags

His and roGFP2

Expression

Bacterial

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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PJEBAN6

Plasmid

#203684

Purpose

Expresses red fluorescent marker DsRed-Express in Listeria monocytogenes

Depositor

Tine Rask Licht

Article

Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

Insert

DsRed-Express

Expression

Bacterial

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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GST-OPTN

Plasmid

#171424

Purpose

For the purifcation of OPTN protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

OPTN (OPTN Human)

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-2

Plasmid

#72677

Purpose

Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.

Depositor

Csaba Pál

Article

Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

Insert

mutL E32K

Tags

none

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Promoter

pL promoter

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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BAZ2AA-c002

Plasmid

#53623

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

BAZ2AA

Tags

His-6-TEV

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Promoter

T7

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pCW-LIC-N8C_Fab3a

Plasmid

#203689

Purpose

Expresses N8C_Fab3a

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab3a

Expression

Bacterial

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET-CjCas9

Plasmid

#89754

Purpose

Expression of CjCas9 with His tag in E.coli

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

CjCas9

Use

CRISPR

Tags

6XHis, HA, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Hia5-OPT1

Plasmid

#231968

Purpose

Expresses DNA adenine N6-methyltransferase Hia5 in Escherichia coli (E.coli) T7 Express lysY/Iq

Depositor

Schahram Akbarian

Article

Peter et al Cell Rep Methods. 2024 Nov 25:100911. do

Insert

DNA adenine N6-methyltransferase Hia5

Tags

Histidine tag

Expression

Bacterial

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2-sfGFP

Plasmid

#88921

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-proinsulin-msGFP2

Plasmid

#160466

Purpose

Encodes a fusion of human proinsulin and the monomeric superfolder GFP2

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

proinsulin-msGFP2

Expression

Bacterial

Promoter

T5

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_mCherry_BamUK

Plasmid

#114596

Purpose

Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

His6 and mCherry

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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His-lsMSP1D1

Plasmid

#240151

Purpose

Expression of the MSP 'His-lsMSP1D1'

Depositor

Lise Arleth

Article

Tidemand et al Biochim Biophys Acta Biomembr. 2022 Jun

Insert

His-lsMSP1D1

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB3m

Plasmid

#120289

Purpose

Produce human recombinant mTGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

monomeric Transforming Growth Factor-β3

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB2k-GFP

Plasmid

#35345

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pTlpA36-Wasabi

Plasmid

#86115

Purpose

mWasabi reporter gene gated by TlpA36

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA36
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A, P60L, D135V, K187R, K202I, L208Q

Promoter

pTlpA and pTlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKD237-3a-2

Plasmid

#90957

Purpose

Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23100, Bba_J23105, and PphsA

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-CD63

Plasmid

#194989

Purpose

overexpress CD63

Depositor

Angelique Bordey

Insert

GFP-CD63

Expression

Bacterial

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pKD4_mCherry

Plasmid

#187386

Purpose

To chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRT

Depositor

Roi Avraham

Insert

mCherry

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT11

Plasmid

#200991

Purpose

pLv1-pUC18R6KT-miniTn7-Gm, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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