30,567 results

1. pQCascade-array1

   Plasmid

   #140623

   Purpose

   Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEVOL-ABK

   Plasmid

   #126035

   Purpose

   Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:

   Inserts

   tRNA synthetase 1
   tRNA synthetase 2
   tRNA for TAG codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L274M, C313A, Y349F

   Promoter

   araBAD promoter, glnS promoter, and proK promoter

   Available Since

   Sept. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCascade-IS1

   Plasmid

   #140624

   Purpose

   Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEVA3b67Rb

   Plasmid

   #58311

   Purpose

   Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET-mEosEM

   Plasmid

   #132708

   Purpose

   application in Correlative light-electron microscopy

   Depositor

   Pingyong Xu

   Article

   Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   mEosEM
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPSU100

   Plasmid

   #173835

   Purpose

   Expresses 100 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABPACIL1bQRS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE-60 Grx1-roGFP2-His

   Plasmid

   #64799

   Purpose

   Bacterial expression of His-tagged Grx1-roGFP2

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Tags

   His and roGFP2

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-32a(+)-TGFB3

   Plasmid

   #120288

   Purpose

   Produce human recombinant TGFB3 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)
   

   Use

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDB179-DepoPETase

   Plasmid

   #218702

   Purpose

   Encodes codon optimized DepoPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   DepoPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-smURFP-RBS-HO-1

    Plasmid

    #80341

    Purpose

    Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    smURFP
    HO-1
    

    Use

    Tags

    6 Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBSV2_P0026-mCherryBb

    Plasmid

    #118239

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0026 promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    bb0026 promoter driven mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNP1954

    Plasmid

    #222730

    Purpose

    pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_GNAI3_G-alpha

    Plasmid

    #110014

    Purpose

    Protein expression and purification of GNAI3_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAI3_G-alpha (GNAI3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSP1

    Plasmid

    #20060

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of deletion mutant (1-43) of human…

    Promoter

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-4T1_ATG4B_BamHI_NotI

    Plasmid

    #190862

    Purpose

    Plasmid for the expression and purification of of ATG4B. Internal reference: SMC1392

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    ATG4B (ATG4B Human)
    

    Use

    Tags

    GST, Thrombin Cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGGF_RT_FAST_RFP

    Plasmid

    #136979

    Purpose

    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

    Depositor

    Klaus Theres

    Article

    Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

    Insert

    seed coat marker RFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

    Promoter

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRE-Tn7-145

    Plasmid

    #118561

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUltra-sY

    Plasmid

    #82417

    Purpose

    Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Methanococcus jannaschii aaRS for sulfotyrosine
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…

    Promoter

    tacI

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a_Pro-IL-1β

    Plasmid

    #214340

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1B (IL1B Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

    Plasmid

    #213024

    Purpose

    Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

    Depositor

    Martin S. Taylor, Oliver Weichenrieder

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (238-1061)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7 / Lac

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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