33,434 results
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Plasmid#106386PurposeConstitutive trc promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
PIA586
Plasmid#104399Purposevector for expression of single E.coli RNAP subunitDepositorInsertrpoD
TagsHis6ExpressionBacterialPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBADTrfp
Plasmid#99382PurposeThis promoter system provided the highest RFP expression upon inductionDepositorInsertRFP
ExpressionBacterialAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ1415
Plasmid#84828PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 intronsDepositorInsertC. elegans codon optimized GFP
ExpressionBacterial and WormPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-Dendra2
Plasmid#82436PurposeDendra2 expression plasmidDepositorInsertDendra2
TagsGST tagExpressionBacterialPromotertacAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-NLyn-Venus-Zdk1
Plasmid#81042Purposemammalian expression of plasma-membrane anchored Zdk1DepositorInsertZdk1
Tags6x His tag, FLAG, NLyn, and mVenusExpressionBacterial, Insect, and Mamm…Available sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FIP3
Plasmid#74753PurposeExpressed sfGFP-tagged FIP3 in E. coli for protein productionDepositorInsertRab11 family interacting protein 3 (RAB11FIP3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialPromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
MBP-ch-TOG-His6
Plasmid#69108PurposeFor bacterial expression of recombinant human ch-TOG tagged with MBP and His.DepositorInsertColonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
TagsHis6 and MBPExpressionBacterialAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJH65
Plasmid#67273PurposeConjugally transferable red recombinogenic plasmidDepositorInserttetA
UseRed recombinase, temperature sensitiveExpressionBacterialPromotertetA promoter of plasmid pACYC184 originAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HRAS
Plasmid#55653Purposeexpression of mouse HRASDepositorAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorPublicationInsertλCI-β-flap fusion
ExpressionBacterialPromoterlac UV5Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorPublicationInsertalpha NTD-Beta flap fusion
ExpressionBacterialPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-Ec.coaA
Plasmid#50386PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.DepositorInsertPanK
Tags6XHis-tagExpressionBacterialPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTD103aiiA
Plasmid#48884PurposeNegative feedback plasmid from oscillatorDepositorInsertaiiA and LuxR
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TRBP
Plasmid#50351Purposebacterial expression of TRBPDepositorAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQF-lacZ
Plasmid#48094PurposeCumate-inducible expression of lacZDepositorInsertE. coli lacZ
ExpressionBacterialPromoterPQ5Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRF-PhlF
Plasmid#49367DepositorInsertPhlF repressor
UseSynthetic BiologyExpressionBacterialAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)
Plasmid#48285DepositorTypeEmpty backboneTagsHis6ExpressionBacterialAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTDpelB-NTwinStrep
Plasmid#45940Purposeuse to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneTags6xHis, PelB, and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTD-NStrepHis
Plasmid#45936Purposeuse to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tagDepositorTypeEmpty backboneTags6xHis and Strep IIExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by