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31,059 results

Showing: 2001 - 2020 of 31059 results

  1. (SUMO)10-(SIM)5

    Plasmid
    #126866
    Purpose
    Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126866-plasmid-map-sequence-id-263307

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  3. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Article
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Expression
    Bacterial
    Available Since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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  8. pAraH6HATT

    Plasmid
    #63901
    Purpose
    For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Tags
    6xHis and HA tag
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    63901-plasmid-map-sequence-id-136457

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  9. His-DNPH1

    Plasmid
    #203224
    Purpose
    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    DNPH1 (DNPH1 Human)
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203224-plasmid-map-sequence-id-400075

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  10. SPLICSFAST-ER-PO-P2A

    Plasmid
    #214894
    Purpose
    Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-ER-PO-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214894-plasmid-map-sequence-id-423480

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  12. pTCF37 HLA-B*35:01

    Plasmid
    #180450
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*35:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    180450-plasmid-map-sequence-id-358091

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Showing: 2001 - 2020 of 31059 results