30,567 results

1. pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)

   Plasmid

   #197660

   Purpose

   Expresses GFP under the control of J23100 and BCD2

   Depositor

   Eric Young

   Article

   Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. His6tev CBP HAT (1324-1700)

   Plasmid

   #99340

   Purpose

   bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

   Depositor

   Peter Wright

   Article

   Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

   Insert

   mouse CBP HAT domain (1324-1700) (Crebbp Mouse)
   

   Use

   Tags

   His6tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-HsSENP1

   Plasmid

   #71465

   Purpose

   Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 1 (SENP1 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pRK172-6xHis-0N4R-P301S-tau-HiBiT

   Plasmid

   #187835

   Purpose

   Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

   Depositor

   William McEwan

   Article

   Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

   Insert

   6XHis-0N4R-P301S-tau-HiBiT
   

   Use

   Tags

   6xHistidine and HiBiT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGLO-Pink-SHE

   Plasmid

   #220781

   Purpose

   Expresses a raspberry chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)

   Depositor

   Angela Leu

   Insert

   raspberry chromoprotein (mRFP1)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLPT20

   Plasmid

   #85460

   Purpose

   Repressilator with integrated reporter

   Depositor

   Johan Paulsson

   Article

   Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

   Inserts

   mCfp
   mVenus
   tetR
   lacI
   cI
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   Promoter

   PLlac, PLtet, PR, and PRNAI

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZac2.1 gfaABC1D-BioID2-BioID2-HA

   Plasmid

   #176740

   Purpose

   Expresses the promiscuous small biotin ligase, BioID2, in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWUR702

   Plasmid

   #53079

   Purpose

   Inducible TtAgo expression

   Depositor

   John van der Oost

   Article

   Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

   Insert

   TtAgo
   

   Use

   Tags

   Strep-II tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPSU30

   Plasmid

   #173830

   Purpose

   Expresses 30 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   HSTPABPACS100BCBP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPSU10

    Plasmid

    #173827

    Purpose

    Expresses 10 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTarget

    Plasmid

    #214052

    Purpose

    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Target sequence
    

    Use

    Target rna plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNon-target

    Plasmid

    #214053

    Purpose

    Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Non-target sequence
    

    Use

    Non-target rna plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

    Plasmid

    #216421

    Purpose

    Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    3xmyc

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPbU6_hdhfr/yfcu

    Plasmid

    #216422

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a_Pro-IL-33

    Plasmid

    #214342

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL33 (IL33 Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBbS5k-RFP

    Plasmid

    #35285

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJL1-CRM197-4xDQNAT

    Plasmid

    #128395

    Purpose

    In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Diphtheria toxin
    

    Use

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    Inactivating G52E mutation

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-28a-hPKM1

    Plasmid

    #44241

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

    Insert

    Pyruvate Kinase M1 (PKM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCCM’

    Plasmid

    #162709

    Purpose

    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    p15A origin replaced with high-copy colE1 origin

    Promoter

    PLtet0-1 promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-scFv-T

    Plasmid

    #67843

    Purpose

    pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

    Depositor

    Joanna Bereta

    Article

    Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

    Insert

    antibody 1D8 light kappa chain
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge