30,576 results

1. pQE30-His-AsnRS

   Plasmid

   #124105

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Asparaginyl-tRNA ligase (asnS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC18T-mini-Tn7T-Zeo-gfpmut3

   Plasmid

   #65037

   Purpose

   ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   gfpmut3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

   Plasmid

   #104394

   Purpose

   Yeast MCP-GFP expression

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   MCP-NLS-2xyeGFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   MCP fused to NLS(SV40) and 2x yeast codon optimiz…

   Promoter

   CYC1

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB223

   Plasmid

   #164127

   Purpose

   New measurement standard

   Depositor

   Christopher Voigt

   Article

   Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

   Insert

   YFP-PP7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAWP78-PVCpnf_pvc13-Ad5RGDPK7

   Plasmid

   #198291

   Purpose

   Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-Ad5RGDPK7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQE60-EFG-His

   Plasmid

   #124126

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Elongation factor G (fusA Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET3a aSyn murine

   Plasmid

   #108865

   Purpose

   Expresses murine alpha synuclein

   Depositor

   Gabriele Kaminski Schierle

   Insert

   alpha synuclein (Snca Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMV306hsp+Lux

   Plasmid

   #26159

   Depositor

   Siouxsie Wiles, Brian Robertson

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   Bacterial luciferase operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Promoter

   Available Since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSET-iGluSnFR4f

   Plasmid

   #234444

   Purpose

   Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f
   

   Use

   Tags

   6xHis-Xpress epi

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSKB3.MPN348

    Plasmid

    #11437

    Depositor

    Sung-Hou Kim

    Article

    Chen et al Proteins. 2004 Sep 1. 56(4):839-43.

    Insert

    methenyltetrahydrofolate synthetase
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cys-30nmSAH-SPYcatcher

    Plasmid

    #214839

    Purpose

    This plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Cys-30nmSAH-SPYcatcher
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNP1723

    Plasmid

    #222727

    Purpose

    sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRIM3-rfp

    Plasmid

    #74073

    Purpose

    GentR version of pRIM2 carrying rfp

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    rfp
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET32a-TRXtag

    Plasmid

    #11516

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    None - thioredoxin is vector encoded (trxA )
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    vector modified; removed N-terminal His tag by di…

    Promoter

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pET-28a(+)-FGF2-K128N

    Plasmid

    #120284

    Purpose

    Produce human recombinant FGF2 K128N in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Basic fibroblast growth factor 2 (FGF2 Synthetic, Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCardinal-pBAD

    Plasmid

    #54800

    Purpose

    Localization: Protein Expression Vector , Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    6xHis and mCardinal

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pQE60-EFTu-His

    Plasmid

    #124127

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor Tu (tufB Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LB322 (Bcl-2 from ATG to -3934)

    Plasmid

    #15381

    Depositor

    Linda Boxer

    Article

    Heckman et al J Biol Chem. 2000 Mar 3. 275(9):6499-508

    Insert

    B-cell lymphoma/leukemia-2 (BCL2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Bacterial

    Mutation

    Bcl-2 fragment from ATG to -3934 with both P1 and…

    Promoter

    Available Since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNP1830

    Plasmid

    #222728

    Purpose

    pNP561 - T5 Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28A-Rab8A

    Plasmid

    #228880

    Purpose

    Expression of His-tagged Rab8A in E. coli

    Depositor

    Sebastian Mathea

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    RAB8A (RAB8A Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    residues 1-6 and 177-207 missing

    Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge